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Table 1.

FoxD3 rescue of axis formation in embryos injected with VP16-FoxD3 or FoxD3MO

NNormal n (%)Axial defects n (%)
Uninjected 85 83 (98) 2 (2) 
VP16-FoxD3 44 12 (27) 32 (73) 
VP16-FoxD3+FoxD3 61 53 (87) 8 (13) 
FoxD3MO 38 8 (21) 30 (79) 
FoxD3MO+FoxD3(−utr) 54 49 (91) 5 (9) 
FoxD3MO+FoxD3(+utr) 49 16 (33) 33 (67) 
Mismatch MO 42 41 (98) 1 (2) 
FoxD3 50 46 (92) 4 (8) 
NNormal n (%)Axial defects n (%)
Uninjected 85 83 (98) 2 (2) 
VP16-FoxD3 44 12 (27) 32 (73) 
VP16-FoxD3+FoxD3 61 53 (87) 8 (13) 
FoxD3MO 38 8 (21) 30 (79) 
FoxD3MO+FoxD3(−utr) 54 49 (91) 5 (9) 
FoxD3MO+FoxD3(+utr) 49 16 (33) 33 (67) 
Mismatch MO 42 41 (98) 1 (2) 
FoxD3 50 46 (92) 4 (8) 

To determine the specificity of VP16-FoxD3 and FoxD3MO, FoxD3 RNA was coinjected with VP16-FoxD3 or FoxD3MO to rescue axis formation. At the 4-cell stage, both dorsal blastomeres were injected with VP16-FoxD3 (0.5 ng)or FoxD3MO (25 ng) alone, or in combination with FoxD3 RNA (25 pg),and axis formation was assessed at the tadpole stage (stage 35). As controls,both dorsal blastomeres were injected with mismatch MO (25 ng) or FoxD3 RNA. Embryos in the `axial defects' class lacked head structures (eyes or cement gland absent) and had greatly reduced trunk and tail, whereas embryos in the `normal' class had near-normal head (eyes and cement gland present), trunk and tail structures. See Fig. S4 in the supplementary material for representative examples of phenotypic classes. N,total number of embryos; n, number of embryos in phenotypic class; %,percentage of embryos in phenotypic class.

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