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Table 2.

Dauer assays

Summary of dauer phenotypes
Genotype 20°C 25°C 27°C
N2   +   +   +  
ifta-2(tm1724)  Daf-d   Daf-d   Daf-c  
osm-5(m184)  Daf-d   Daf-d   Daf-c  
daf-10(e1387)  Daf-d   Daf-d   Daf-c  
daf-2(e1370)  +   Daf-c   Daf-c  
daf-16(mu86)  Daf-d   Daf-d   +  
ifta-2(tm1724);daf-16(mu86)  Daf-d   Daf-d   +  
Summary of dauer phenotypes
Genotype 20°C 25°C 27°C
N2   +   +   +  
ifta-2(tm1724)  Daf-d   Daf-d   Daf-c  
osm-5(m184)  Daf-d   Daf-d   Daf-c  
daf-10(e1387)  Daf-d   Daf-d   Daf-c  
daf-2(e1370)  +   Daf-c   Daf-c  
daf-16(mu86)  Daf-d   Daf-d   +  
ifta-2(tm1724);daf-16(mu86)  Daf-d   Daf-d   +  

Dauer assays were performed at three temperatures at least three independent times with approximately 50-100 animals per experiment. N2, daf-2(e1370), daf-10(e1387) and daf-16(m86) served as controls. daf-c, dauer constitutive; daf-d, dauer defective; +, wild type for dauer formation

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