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Table 1.

Temporal image correlation analysis

Protein Immobile proteins (%)
Integrin   88*±5  
Paxillin   74±14  
FAK   74±15  
Talin   63±16  
Vinculin   66±20  
α-actinin   56±21  
Actin   28±9  
Protein Immobile proteins (%)
Integrin   88*±5  
Paxillin   74±14  
FAK   74±15  
Talin   63±16  
Vinculin   66±20  
α-actinin   56±21  
Actin   28±9  

The percentage of immobile proteins was calculated based on the amplitude of the temporal auto-correlation function and the amplitude of the auto-correlation-function offset due to the immobile component (Wiseman and Brown et al., 2004). All measurements were taken in regions of the cell that showed visible adhesions except for integrin measurements (because adhesions were not visible)

*

For immobile fractions >90% it is difficult to fit the temporal correlation function because it does not decay very much. When the functions could not be fit, an immobile fraction of 90% (rather than 100%) was assumed, although in some cases the fraction might be even higher. Thus, 88% is the minimum percentage of immobile integrin. Errors are s.d.

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