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Table 3.

Marker gene expression in transgenic embryos

n% Normal% Reduced% No stain% Ectopic
Control-Raldh 40 83 15 2.5 
Mesp-VP16-Raldh 33 36 42 15 
Control-Hand-like 161 94 1.2 4.3 
Mesp-VP16-Hand-like 71 39 61 
n% Normal% Reduced% No stain% Ectopic
Control-Raldh 40 83 15 2.5 
Mesp-VP16-Raldh 33 36 42 15 
Control-Hand-like 161 94 1.2 4.3 
Mesp-VP16-Hand-like 71 39 61 

Embryos were only scored if they displayed normal morphology, scoring refers to staining pattern. `Reduced' indicates very low levels of staining in comparison with controls (for example, see Fig. 5F). For Raldh,`Ectopic' indicates staining in the head region, this staining was always very weak. For Hand-like, `Ectopic' indicates staining in or near the tail region (see Fig. 5E).

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