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Table 2.

Transgenic juvenile heart phenotypes




n

% Normal

% Mild disruption

% Severe disruption

% No heart

% Ectopic heart
Mesp-lacZ or GFP 230 74 10 14 
Mesp-VP16 120 49 29 13 
MyoD-VP16
 
202
 
19
 
15
 
34
 
32
 
0
 



n

% Normal

% Mild disruption

% Severe disruption

% No heart

% Ectopic heart
Mesp-lacZ or GFP 230 74 10 14 
Mesp-VP16 120 49 29 13 
MyoD-VP16
 
202
 
19
 
15
 
34
 
32
 
0
 

`Mild disruption' indicates improper beat indicative of minor myocardial abnormalities. `Severe disruption' generally indicates major to nearly complete loss of beating myocardial tissue. The `No heart' category includes some juveniles in which a pericardial chamber was present but no beating myocardial tissue was observed.

Data for the Mesp-VP16 and MyoD-VP16 juveniles represent two trials, whereas the Mesp-lacZ/GFP control data is from four trials. Many of the transgenic Mesp-VP16 juveniles died soon after metamorphosis or displayed general morphological disruptions. This may be due to the loss of Raldh expression (and the subsequent interference with retinoic acid synthesis) in fully penetrant Mesp-VP16 juveniles (see Table 3).

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