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Table 1.

Yeast two-hybrid interaction assays: αT-catenin specifically interacts with plakophilin-1, -2 and -3

Number Bait plasmid Prey plasmid Interaction
A     
   1   pGBT9-α-catulin   pGADT7-hPKP3   –  
   2   pGBT9-αE-catenin   pGADT7-hPKP3   –  
   3   pGBT9-αN-catenin   pGADT7-hPKP3   –  
   4   pGBT9-αT-catenin   pGADT7-hPKP3   +  
   5   pGBKT7-αT-catenin   pGADT7-hPKP3   +  
   6   pGBKT7-hPKP3   pGADT7-αT-catenin   +  
   7   pGBKT7-hPKP3   pGAD424-αE-catenin   –  
   8   pGBKT7-hPKP3   pGAD424-αN-catenin   –  
B     
   9   peGBKT7-hαT-catenin   pGAD424-β-catenin(N-term)   +  
   10   peGBKT7-hαT-catenin   pGADT7-hPKP1   +  
   11   pAS2-hPKP2   pGADT7-αT-catenin   +  
   12   pAS2-hPKP2   pGAD424-αE-catenin   –  
C     
   13   pGBKT7-p0071 head ΔC2   pGADT7-αT-catenin   –  
   14   pGBKT7-p0071 head ΔN1   pGADT7-αT-catenin   –  
   15   pdGBKT7-hδ-catenin short   pGADT7-αT-catenin   –  
   16   peGBKT7-hαT-catenin   pdGADT7-hδ-catenin short   –  
   17   pGBKT7-p120ctn-3AC   pGADT7-αT-catenin   –  
   18   pGBKT7-mARVCF CM   pGADT7-αT-catenin   –  
Number Bait plasmid Prey plasmid Interaction
A     
   1   pGBT9-α-catulin   pGADT7-hPKP3   –  
   2   pGBT9-αE-catenin   pGADT7-hPKP3   –  
   3   pGBT9-αN-catenin   pGADT7-hPKP3   –  
   4   pGBT9-αT-catenin   pGADT7-hPKP3   +  
   5   pGBKT7-αT-catenin   pGADT7-hPKP3   +  
   6   pGBKT7-hPKP3   pGADT7-αT-catenin   +  
   7   pGBKT7-hPKP3   pGAD424-αE-catenin   –  
   8   pGBKT7-hPKP3   pGAD424-αN-catenin   –  
B     
   9   peGBKT7-hαT-catenin   pGAD424-β-catenin(N-term)   +  
   10   peGBKT7-hαT-catenin   pGADT7-hPKP1   +  
   11   pAS2-hPKP2   pGADT7-αT-catenin   +  
   12   pAS2-hPKP2   pGAD424-αE-catenin   –  
C     
   13   pGBKT7-p0071 head ΔC2   pGADT7-αT-catenin   –  
   14   pGBKT7-p0071 head ΔN1   pGADT7-αT-catenin   –  
   15   pdGBKT7-hδ-catenin short   pGADT7-αT-catenin   –  
   16   peGBKT7-hαT-catenin   pdGADT7-hδ-catenin short   –  
   17   pGBKT7-p120ctn-3AC   pGADT7-αT-catenin   –  
   18   pGBKT7-mARVCF CM   pGADT7-αT-catenin   –  
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