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Table 2.

Penetrance of phenotypic manifestations following compromising tac-1 and/or zyg-8 function

Embryonic genotype Meiotic defects* (in %) Distance from posterior cortex (in μm) Cleavage-furrow ingression (in %)
N2 (wild-type)   0 (8)   18.9 (8)   100 (8)  
tac-1(RNAi)§  8 (25)   2.9 (21)   100 (26)  
zyg-8(or484)   26§ (29)   17.7 (29)   86 (29)  
tac-1(RNAi) zyg-8(or484)   82 (17)   1.7 (17)   0 (18)  
Embryonic genotype Meiotic defects* (in %) Distance from posterior cortex (in μm) Cleavage-furrow ingression (in %)
N2 (wild-type)   0 (8)   18.9 (8)   100 (8)  
tac-1(RNAi)§  8 (25)   2.9 (21)   100 (26)  
zyg-8(or484)   26§ (29)   17.7 (29)   86 (29)  
tac-1(RNAi) zyg-8(or484)   82 (17)   1.7 (17)   0 (18)  

Not all embryos could be analyzed for each trait. The distance from the posterior cortex in tac-1(RNAi) embryos (average of 2.9 μm; ±s.d.=1.1) is statistically different from that in tac-1(RNAi) zyg-8(or484) embryos (average of 1.7 μm; ±s.d.=1.0). Student's paired t-test; P<0.003

The number of embryos scored is given in parentheses

*

Percentage of embryos with two or more female pronuclei. A few other embryos exhibited larger polar bodies but no extra female pronucleus and were not included here

Average distance between the posterior cortex and the posterior rim of the male pronucleus at nuclear envelope breakdown (NEBD)

Percentage of embryos in which the cleavage furrow ingressed to bisect the spindle

§

The female pronucleus is often initially smaller than the male pronucleus in zyg-8(or484) mutant embryos, possibly reflecting delayed progression through the female meiotic divisions

In 14% of zyg-8(or484) mutant embryos, the cleavage furrow ingressed initially before regressing in the end, probably because of the very unstable spindle positioning that preceded

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