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Table 1.

Summary of cellular velocities

Protein Challenge Comments Velocity* (μm/second) n
GFP-Myo52   None   N-terminally GFP-tagged Myo52 expressed from chromosome in myo52Δ background   0.502±0.09   403  
GFP-Myo52   None   N-terminally GFP-tagged Myo52 expressed from multicopy plasmid in myo52Δ background   0.508±0.07   362  
Myo52-GFP   None   C-terminally GFP-tagged Myo52 expressed from endogenous locus   0.511±0.06   972  
Myo52-GFP  myo51Δ strain   Gene encoding Myo51, a second myosin V, deleted from S. pombe genome   0.518±0.08   216  
GFP-Crn1   None   Actin-patch marker   0.28±0.04   147  
Myo52-GFP   Nitrogen-starved cells   Disorganised actin cytoskeleton   0   —  
Myo52-GFP   Glucose-starved cells   Cells subjected to nutrient stress   No effect   —  
Myo52-GFP   Cells in sorbitol (1.2 M)   Cells subjected to osmotic stress   No effect   —  
Myo52-GFP   CBZ (25 μg/ml)   No microtubules   0.507±0.07   132  
Myo52-GFP   Latruncullin A (1 μM)   No actin filaments   0   —  
Myo52-GFP   Latruncullin A (20 μM)   No actin structures   0   —  
Myo52-GFP  cdc3-124 strain at 36°C   No F-actin   0   —  
Myo52-GFP  cdc8-110 strain at 36°C   No actin filaments   0   —  
Myo52-GFP  for3Δ strain   No interphase actin filaments   0   —  
GFP-Crn1   FCCP (10 nM)   Reduced cellular [ATP]   0.265±0.04   104  
Myo52-GFP   FCCP (10 nM)   Reduced cellular [ATP]   0   —  
GFP-Myo52ΔIQ   None   Myo52 lacking neck region in myo52Δ background   0.522±0.14   233  
Protein Challenge Comments Velocity* (μm/second) n
GFP-Myo52   None   N-terminally GFP-tagged Myo52 expressed from chromosome in myo52Δ background   0.502±0.09   403  
GFP-Myo52   None   N-terminally GFP-tagged Myo52 expressed from multicopy plasmid in myo52Δ background   0.508±0.07   362  
Myo52-GFP   None   C-terminally GFP-tagged Myo52 expressed from endogenous locus   0.511±0.06   972  
Myo52-GFP  myo51Δ strain   Gene encoding Myo51, a second myosin V, deleted from S. pombe genome   0.518±0.08   216  
GFP-Crn1   None   Actin-patch marker   0.28±0.04   147  
Myo52-GFP   Nitrogen-starved cells   Disorganised actin cytoskeleton   0   —  
Myo52-GFP   Glucose-starved cells   Cells subjected to nutrient stress   No effect   —  
Myo52-GFP   Cells in sorbitol (1.2 M)   Cells subjected to osmotic stress   No effect   —  
Myo52-GFP   CBZ (25 μg/ml)   No microtubules   0.507±0.07   132  
Myo52-GFP   Latruncullin A (1 μM)   No actin filaments   0   —  
Myo52-GFP   Latruncullin A (20 μM)   No actin structures   0   —  
Myo52-GFP  cdc3-124 strain at 36°C   No F-actin   0   —  
Myo52-GFP  cdc8-110 strain at 36°C   No actin filaments   0   —  
Myo52-GFP  for3Δ strain   No interphase actin filaments   0   —  
GFP-Crn1   FCCP (10 nM)   Reduced cellular [ATP]   0.265±0.04   104  
Myo52-GFP   FCCP (10 nM)   Reduced cellular [ATP]   0   —  
GFP-Myo52ΔIQ   None   Myo52 lacking neck region in myo52Δ background   0.522±0.14   233  
*

Directed cytoplasmic movements only.

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