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Table 1.

Quantitative analyses of marker gene expression

Targets
InjectedGli1Gli2Gli3Ptc1Ptc2NcamNgn1aNeuroDNtub
Stage 12          
Gli1+MO-C  10.1* 0.5* 36.5* 135.2* 0.6* 1.5* 1.0* 14.0* 
Gli1+MO-2   0.7 0.8 0.6 0.8 1.0 1.1 0.9 
Gli1+MO-3  1.1  0.5 0.3 1.3 1.1 0.7 0.4 
Gli2+MO-C 6.3*  0.5* 12.2* 29.2* 1.0* 1.6* 0.5* 19.8* 
Gli2+MO-1   0.7 0.7 0.6 0.6 0.6 0.3 0.5 
Gli2+MO-3 0.4   0.5 0.6 2.1 1.4 1.0 0.4 
Gli3+MO-C 0.9* 13.2*  10.3* 18.2* 1.4* 4.8* 2.7* 4.6* 
Gli3+MO-1  0.5  0.9 0.6 1.0 0.4 0.2 0.7 
Gli3+MO-2 0.8   0.5 0.6 0.7 1.2 0.6 0.3 
Stage 14          
Gli1+MO-C  21.0* 1.0* 39.5* 84.5* 0.4* 7.5* 7.9* 4.7* 
Gli1+MO-2   0.7 1.0 0.7 0.9 0.8 0.7 0.7 
Gli1+MO-3  0.9  0.8 0.8 1.3 1.8 0.7 0.5 
Gli2+MO-C 20.1*  2.7* 10.4* 23.0* 1.3* 15.1* 1.4* 28.8* 
Gli2+MO-1   0.4 0.5 0.7 0.6 0.6 1.5 0.4 
Gli2+MO-3 0.3   0.3 0.2 0.6 0.6 2.8 0.1 
Gli3+MO-C 6.3* 28.4*  10.6* 12.5* 2.0* 16.3* 3.7* 8.4* 
Gli3+MO-1  0.4  0.5 0.6 0.5 0.4 0.8 0.2 
Gli3+MO-2 0.4   0.5 1.2 0.7 0.4 0.7 0.1 
Stage 14          
GLI1+MO-C 6.9* 5.2* 1.3* 20.9* 64.5* 1.2* 2.6* 0.5* 2.6* 
GLI1+MO-1  0.8 3.5 0.1 0.2 2.0 0.5 1.9 0.7 
GLI1+MO-2 1.1  1.3 0.9 0.8 5.6 2.5 4.0 1.6 
GLI1+MO-3 0.7 0.7  >0 >0 2.4 1.1 3.3 0.2 
GLI2+MO-C 19.7* 5.5* 2.0* 18.3* 17.4* 6.3* 4.3* 16.5* 22.8* 
GLI2+MO-1  0.9 1.2 0.5 0.8 0.4 1.2 >0 0.3 
GLI2+MO-2 1.2  1.9 2.2 1.0 0.4 1.4 >0 0.2 
GLI2+MO-3 1.0 1.5  0.8 1.2 0.9 5.2 2.0 0.3 
GLI3+MO-C 9.5* 22.2* 3.7* 10.6* 4.9* 4.1* 15.1* 4.6* 34.8* 
GLI3+MO-1  0.3 0.3 0.4 1.3 1.2 0.5 0.1 0.2 
GLI3+MO-2 0.9  0.3 0.7 1.4 2.4 0.5 0.2 0.7 
GLI3+MO-3 1.0 0.8  0.9 1.8 0.4 0.4 0.8 0.7 
Targets
InjectedGli1Gli2Gli3Ptc1Ptc2NcamNgn1aNeuroDNtub
Stage 12          
Gli1+MO-C  10.1* 0.5* 36.5* 135.2* 0.6* 1.5* 1.0* 14.0* 
Gli1+MO-2   0.7 0.8 0.6 0.8 1.0 1.1 0.9 
Gli1+MO-3  1.1  0.5 0.3 1.3 1.1 0.7 0.4 
Gli2+MO-C 6.3*  0.5* 12.2* 29.2* 1.0* 1.6* 0.5* 19.8* 
Gli2+MO-1   0.7 0.7 0.6 0.6 0.6 0.3 0.5 
Gli2+MO-3 0.4   0.5 0.6 2.1 1.4 1.0 0.4 
Gli3+MO-C 0.9* 13.2*  10.3* 18.2* 1.4* 4.8* 2.7* 4.6* 
Gli3+MO-1  0.5  0.9 0.6 1.0 0.4 0.2 0.7 
Gli3+MO-2 0.8   0.5 0.6 0.7 1.2 0.6 0.3 
Stage 14          
Gli1+MO-C  21.0* 1.0* 39.5* 84.5* 0.4* 7.5* 7.9* 4.7* 
Gli1+MO-2   0.7 1.0 0.7 0.9 0.8 0.7 0.7 
Gli1+MO-3  0.9  0.8 0.8 1.3 1.8 0.7 0.5 
Gli2+MO-C 20.1*  2.7* 10.4* 23.0* 1.3* 15.1* 1.4* 28.8* 
Gli2+MO-1   0.4 0.5 0.7 0.6 0.6 1.5 0.4 
Gli2+MO-3 0.3   0.3 0.2 0.6 0.6 2.8 0.1 
Gli3+MO-C 6.3* 28.4*  10.6* 12.5* 2.0* 16.3* 3.7* 8.4* 
Gli3+MO-1  0.4  0.5 0.6 0.5 0.4 0.8 0.2 
Gli3+MO-2 0.4   0.5 1.2 0.7 0.4 0.7 0.1 
Stage 14          
GLI1+MO-C 6.9* 5.2* 1.3* 20.9* 64.5* 1.2* 2.6* 0.5* 2.6* 
GLI1+MO-1  0.8 3.5 0.1 0.2 2.0 0.5 1.9 0.7 
GLI1+MO-2 1.1  1.3 0.9 0.8 5.6 2.5 4.0 1.6 
GLI1+MO-3 0.7 0.7  >0 >0 2.4 1.1 3.3 0.2 
GLI2+MO-C 19.7* 5.5* 2.0* 18.3* 17.4* 6.3* 4.3* 16.5* 22.8* 
GLI2+MO-1  0.9 1.2 0.5 0.8 0.4 1.2 >0 0.3 
GLI2+MO-2 1.2  1.9 2.2 1.0 0.4 1.4 >0 0.2 
GLI2+MO-3 1.0 1.5  0.8 1.2 0.9 5.2 2.0 0.3 
GLI3+MO-C 9.5* 22.2* 3.7* 10.6* 4.9* 4.1* 15.1* 4.6* 34.8* 
GLI3+MO-1  0.3 0.3 0.4 1.3 1.2 0.5 0.1 0.2 
GLI3+MO-2 0.9  0.3 0.7 1.4 2.4 0.5 0.2 0.7 
GLI3+MO-3 1.0 0.8  0.9 1.8 0.4 0.4 0.8 0.7 

Numbers indicate fold change in endogenous gene expression in stage 12 or 14 animal caps with frog Gli or human GLI RNAs and MOs, determined by real-time RT-PCR analyses as calculated by the ΔCT method. Gene expression levels were normalized to EF1α. Changes of at least 40% are in green for activator function and in red for repressor function.

MO-C=Gli`x'+MO-C/uninjected AC.

MO-`x'=Gli`x'+MO-`y'/Gli`x'+MO-C.

*

The action of injected Gli proteins.

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