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Table 1.

IsO expression of lmx1b.1 and lmx1b.2 in offspring of no isthmus heterozygotes

Lmx1b.1
Lmx1b.2
StageNormalAbsent% absentNormalAbsent% absent
One somite 17 0.0 17 0.0 
Five somites 52 10 16.1 33 10 23.3 
Seven somites 30 15 33.3 33 17 34.0 
Twelve somites 63 24 27.6 69 28 28.9 
Eighteen somites 30 10 25.0 15 37.5 
Lmx1b.1
Lmx1b.2
StageNormalAbsent% absentNormalAbsent% absent
One somite 17 0.0 17 0.0 
Five somites 52 10 16.1 33 10 23.3 
Seven somites 30 15 33.3 33 17 34.0 
Twelve somites 63 24 27.6 69 28 28.9 
Eighteen somites 30 10 25.0 15 37.5 

Heterozygous parents were crossed and offspring were fixed at several different stages and examined for expression of lmx1b.1 or lmx1b.2. Absence of expression in 25% of the embryos corresponds to the predicted frequency of no isthmus homozygous offspring.

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