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Table 3.

pro-1(na48) genetic interactions and RNAi phenotypes

(A)Genotype*,% Pro% Pexn
 pro-1(na48) 66 92 
 mpk-1(ga111) 91 162 
 pro-1(na48);mpk-1(ga111) 64 100 98 
(A)Genotype*,% Pro% Pexn
 pro-1(na48) 66 92 
 mpk-1(ga111) 91 162 
 pro-1(na48);mpk-1(ga111) 64 100 98 
(B)Genotype*,§,% Pro% Femn
 pro-1(na48) 58** 160 
 fem-1(hc17) 100 152 
 pro-1(na48); fem-1(hc17) 43 90†† 171 
(B)Genotype*,§,% Pro% Femn
 pro-1(na48) 58** 160 
 fem-1(hc17) 100 152 
 pro-1(na48); fem-1(hc17) 43 90†† 171 
(C)Genotype*,§,‡‡Hermaphrodite % Pro(n)Male % Pro(n)
 pro-1(na48); him-5(e1490) 74(80)§§ 23 (40)  
(C)Genotype*,§,‡‡Hermaphrodite % Pro(n)Male % Pro(n)
 pro-1(na48); him-5(e1490) 74(80)§§ 23 (40)  
(D)Genotype*,§,¶¶% Pro% Glpn
 pro-1(na48) 84 62 
 glp-1(e2141) 100 62 
 pro-1(na48);glp-1(e2141) 100 88 
(D)Genotype*,§,¶¶% Pro% Glpn
 pro-1(na48) 84 62 
 glp-1(e2141) 100 62 
 pro-1(na48);glp-1(e2141) 100 88 
(E)Genotype*,§,***% Pro% Vuln
 pro-1(na48) 75** 60 
 lin-12(n302) 98 522 
 pro-1(na48);lin-12(n302) 68 100 72 
(E)Genotype*,§,***% Pro% Vuln
 pro-1(na48) 75** 60 
 lin-12(n302) 98 522 
 pro-1(na48);lin-12(n302) 68 100 72 
(F)Genotype%Gon†††n
 pro-1(RNAi) 100 122  
 rrf-1(pk1417); pro-1(RNAi) 296  
(F)Genotype%Gon†††n
 pro-1(RNAi) 100 122  
 rrf-1(pk1417); pro-1(RNAi) 296  
(G)Genotype*,‡‡‡% Pro at 20°C% Glp at 20°Cn
 pro-1(na48) 11 82 
 glp-1(e2141) 212 
 pro-1(na48);glp-1(e2141) 31 58 
(G)Genotype*,‡‡‡% Pro at 20°C% Glp at 20°Cn
 pro-1(na48) 11 82 
 glp-1(e2141) 212 
 pro-1(na48);glp-1(e2141) 31 58 
(H)Genotype*,§,§§§% Pro at 20°Cn
 pro-1(na48) 19 208  
 glp-1(ar202) 152  
 pro-1(na48);glp-1(ar202) 72 118  
(H)Genotype*,§,§§§% Pro at 20°Cn
 pro-1(na48) 19 208  
 glp-1(ar202) 152  
 pro-1(na48);glp-1(ar202) 72 118  

n, number of gonad arms; Vul, number of animals scored equals half n.

*

Homozygous pro-1(na48): self progeny of heterozygous parents balanced by mIn1.

Strains marked with unc-79(e1068), grown at 20°C and shifted to 25°C as gravid adults. L1 progeny were synchronized and cultured at 25°C for 72 hours [pro-1(na48)-containing strains] or 48 hours,before fixation and DAPI staining. Pex (pachytene exit defect): mitotic,transition and pachytene nuclei present but no gametes.

Pro here is a Pro/Pex phenotype (see text).

§

Strains marked with unc-4(e120).

Strains grown at 20°C. Synchronized L1 animals were shifted to 25°C and scored after 72 hours (pro-1(na48)-containing strains) or 48 hours.

**

These values are not significantly different (P≫0.05) from the corresponding values two rows directly below (two-sided Fisher exact test).

††

Remaining 10% non-Fem animals were non-Pro, non-Fem.

‡‡

Animals fixed and DAPI stained after 72 hours.

§§

This value is significantly different from the value in the next column(P<2×10−7, based on two-sided Fisher exact test).

¶¶

Strains marked with dpy-17(e164) and grown at 20°C. Synchronized L1 animals were shifted to 25°C.

***

For pro-1(na48)-containing strains, mid-L4s were picked to a fresh plate and fixed and DAPI stained 24 hours later. For unc-4(e120);lin-12(n302), all adults from several mixed-stage plates were scored under Normarki optics for Vul and Pro phenotypes.

†††

Asynchronous adults scored for the Gon phenotype under Nomarski optics.

‡‡‡

Strains homozygous for him-5(e1490), grown at 20°C,synchronized, fixed and DAPI stained after 72 hours for glp-1(e2141);him-5(e1490), and after 96 hours for pro-1(na48)-containing strains.

§§§

Animals grown at 20°C, synchronized, fixed and DAPI-stained after 72 hours for unc-4(e120); glp-1(ar202), and 96 hours for pro-1(na48)-containing strains.

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