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Table 2.

Germ cell counts in pro-1(na48) and pro-1(+) per gonad arm at somatic-and germline-specific developmental time points

Developmental eventGenotypeHours post hatchStage*Distal mitosisTransitionPachyteneGametesProximal mitosisTotal germ nuclein
SPh pro-1(+) 18 eL3 13±2 13 28 
 pro-1(na48) 30 eL3 12±1 12 28 
Initial pro-1(+) 26 L3 51±5 7±3 58 16 
meiosis pro-1(na48) 48-54§ lL4 63±14 13±10 13±17 89 27 
Adult pro-1(+) 35.5 adult 130±8 28±8 59±9 22±5 239 10 
molt pro-1(na48) 54 adult 69±13 14±15 19±22 102 12 
Developmental eventGenotypeHours post hatchStage*Distal mitosisTransitionPachyteneGametesProximal mitosisTotal germ nuclein
SPh pro-1(+) 18 eL3 13±2 13 28 
 pro-1(na48) 30 eL3 12±1 12 28 
Initial pro-1(+) 26 L3 51±5 7±3 58 16 
meiosis pro-1(na48) 48-54§ lL4 63±14 13±10 13±17 89 27 
Adult pro-1(+) 35.5 adult 130±8 28±8 59±9 22±5 239 10 
molt pro-1(na48) 54 adult 69±13 14±15 19±22 102 12 

Average germ cell counts±1 s.d.

n, number of gonad arms scored.

*

Stage: eL3, early L3; lL4, late L4.

Sperm and spermatocytes are included. The number of gametes reported for this time point is equal to the number of spermatocytes plus one-quarter of the number of sperm. At the adult molt, pro-1(+) gonad arms contained an average of 16±3 spermatocytes and 22±22 sperm.

Formation of the hermaphrodite somatic gonad primordium.

§

In pro-1(na48), initial meiosis is variable with respect to somatic developmental stage. Therefore, animals from three time points were pooled (4/15, 16/33 and 7/12 germ lines contained meiotic nuclei at 48, 50 and 54 hours post-hatch off, respectively).

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