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Table 1.

Genetic dose and temperature dependence of pro-1 mutant phenotypes

GenotypeTemperature (°C)Pro* (%)Mig (%)Gametogenesis(%)Abnormal vulva§ (%)nHours to adult molt**Relative rate of development††
pro-1 (+) 25 100 180 35.5 
pro-1(+)/pro-1(na48) 25 100 112 35.5 
pro-1(na48) 25 83 38 100 138 54 0.66 
pro-1(na48)/Df‡‡ 25 57 69 100 54 85 0.42 
pro-1(RNAi)§§ 25 53 73 Pvl 17 122 ND ND 
pro-1(+) 20 100 108 47.5 
pro-1(+)/pro-1(na48) 20 100 126 47.5 
pro-1(na48)¶¶ 20 11 56 100 130 78 0.61 
pro-1(na48)/Df*** 20 20 80 100 Vul 7, Pvl 21 56 ∼110 0.43 
pro-1(+) 15 100 122 78 
pro-1(+)/pro-1(na48) 15 100 118 78 
pro-1(na48) 15 65 66 Pvl 9 114 ∼168 0.46 
pro-1(na48)/Df 15 100 36 Vul 100 28 ∼240 0.33 
GenotypeTemperature (°C)Pro* (%)Mig (%)Gametogenesis(%)Abnormal vulva§ (%)nHours to adult molt**Relative rate of development††
pro-1 (+) 25 100 180 35.5 
pro-1(+)/pro-1(na48) 25 100 112 35.5 
pro-1(na48) 25 83 38 100 138 54 0.66 
pro-1(na48)/Df‡‡ 25 57 69 100 54 85 0.42 
pro-1(RNAi)§§ 25 53 73 Pvl 17 122 ND ND 
pro-1(+) 20 100 108 47.5 
pro-1(+)/pro-1(na48) 20 100 126 47.5 
pro-1(na48)¶¶ 20 11 56 100 130 78 0.61 
pro-1(na48)/Df*** 20 20 80 100 Vul 7, Pvl 21 56 ∼110 0.43 
pro-1(+) 15 100 122 78 
pro-1(+)/pro-1(na48) 15 100 118 78 
pro-1(na48) 15 65 66 Pvl 9 114 ∼168 0.46 
pro-1(na48)/Df 15 100 36 Vul 100 28 ∼240 0.33 

ND, not determined.

*

Mitotic germ cells proximal to gametes. In a separate experiment, 72 hours post-hatch-off, 63% of Pro arms (n=94; 72% Pro) contained both oocytes and sperm and remainder contained only sperm. At 96 hours, 84% of Pro arms (n=70; 71% Pro) contained both oocytes and sperm. Therefore,reduced oogenesis may reflect a delay in the spermatogenesis/oogenesis switch.

Abnormal DTC migration. Defects seen in dorsal, centrifugal or both migrations. Mig and Pro did not correlate nor were they mutually exclusive.

Spermatocytes, sperm and/or oocyte nuclei observed with DAPI or Nomarski.

§

Vul, Vulvaless; Pvl, protruding vulva (versus Evl, abnormally everted vulva).

n=number of gonad arms scored. For vulva phenotypes, the number of animals scored is n/2.

**

Hours to adult molt from the hatch-off (see Materials and methods).

††

Relative development rate: hours from hatch-off to adult molt in wild-type over mutant; ratio gives comparative rates of development at different temperatures.

‡‡

Df = mnDf58. pro-1 (na48)/mnDf58 were synchronized non-GFP progeny of pro-1(na48)/mInl males crossed to mnDf58/mInlhermaphrodites. Pro data alone are inconsistent with pro-1(na48) as a simple reduction of function allele. This inconsistency may result from temperature-sensitive haploinsufficiency of another locus under the deficiency.

§§

Data are reported from a single experiment (see Materials and methods);similar results were obtained from multiple RNAi experiments.

¶¶

Although pro-1 (na48) is only 11% Pro at 20°C, the strain is difficult to maintain because of Emo sterility. Emo: 33%(29/86) of gonad arms,scored by DAPI at 120 hours post-hatch-off.

***

One individual Vul pro-1(na48)/mnDf58 animal displayed the Pro phenotype in both gonad arms.

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