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Table 1.

The occurrence and kinetics of AR and its relationship to cell viability and motility in human sperm

AR (%) Latency of AR after stimulus (min) Time to 90% AR (min) Viability after AR (%) Time of death after AR (min) Motile after AR (%) Cells n
A23187   44.8±2.5   13.4±2.6   8.8±0.9   92.3±1.4   29.5±4.8   0.6±0.2   1230   5  
Spontaneous   2.2±0.7   –   12.0±0.8  5.3±4.3   –   0   2166   5  
Progesterone   10.2±3.9*  16.9±4.5   11.9±0.5  41.1±6.0   7.3±1.6   9.6±2.2   2401   11  
AR (%) Latency of AR after stimulus (min) Time to 90% AR (min) Viability after AR (%) Time of death after AR (min) Motile after AR (%) Cells n
A23187   44.8±2.5   13.4±2.6   8.8±0.9   92.3±1.4   29.5±4.8   0.6±0.2   1230   5  
Spontaneous   2.2±0.7   –   12.0±0.8  5.3±4.3   –   0   2166   5  
Progesterone   10.2±3.9*  16.9±4.5   11.9±0.5  41.1±6.0   7.3±1.6   9.6±2.2   2401   11  

AR was induced by 10 μM A23187, 3 μM progesterone or occurred spontaneously. For each stimulus, the following variables were measured and the mean ± s.e.m. recorded: the percentage of sperm undergoing AR within the duration of the experiment, the latency from time of stimulation to initiation of AR (not recordable for spontaneous AR), the duration from initiation to 90% completion of AR (from normalised fluorescent plots of each cell), the percentage of sperm remaining viable following initiation of AR, the time between AR initiation and cell death, and the percentage of sperm remaining motile following initiation of AR. *Significant compared with percentage of spontaneously occurring AR, P<0.05, Mann-Whitney Test. Significant compared to duration of A23187-induced AR, P<0.05, Mann-Whitney Test. n, number of experiments

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