Results of Cys-shotgun experiments of cardiomyocyte proteome
| Estimated molecular mass (kD) . | Differential labeling (34 kPa and 1 kPa) . | Proteins indentified by mass spectrometry . |
|---|---|---|
| ∼285 | +0.21 | Filamin A and B |
| ∼217 | +0.36 | Non-muscle myosin IIB |
| ∼125 | 0.03 | |
| ∼90 | 0.05 | |
| ∼70 | -0.08 | |
| ∼58 | +0.23 | Pyruvate kinase M1 |
| ∼51 | -0.37 | Vimentin |
| ∼40 | 0.04 | |
| ∼35 | -0.09 | |
| ∼20 | -0.08 |
| Estimated molecular mass (kD) . | Differential labeling (34 kPa and 1 kPa) . | Proteins indentified by mass spectrometry . |
|---|---|---|
| ∼285 | +0.21 | Filamin A and B |
| ∼217 | +0.36 | Non-muscle myosin IIB |
| ∼125 | 0.03 | |
| ∼90 | 0.05 | |
| ∼70 | -0.08 | |
| ∼58 | +0.23 | Pyruvate kinase M1 |
| ∼51 | -0.37 | Vimentin |
| ∼40 | 0.04 | |
| ∼35 | -0.09 | |
| ∼20 | -0.08 |
Analysis of proteins from SDS-PAGE and the difference of fluorescent signal between proteins labeled in cells on hard versus compliant substrates. Proteins showing a difference of >20% in triplicate studies (bold) were subsequently identified by mass spectrometry.