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Table 1.

Eg5 interacts with Nek6 and Nercc1

Eg5 (Gal4 AD)
Gal4 BD 1-361 362-761 762-1057
Nercc1   -   -   +  
Nek6   -   -   +  
Eg5 (Gal4 AD)
Gal4 BD 1-361 362-761 762-1057
Nercc1   -   -   +  
Nek6   -   -   +  

The ability of the Eg5 head (Eg5 1-361), stalk (Eg5 362-761) and tail (Eg5 762-1057) domains to interact with Nek6 or Nercc1 was assessed using the two-hybrid assay (see supplementary material Fig. S1). Gal4 AD/BD, Gal4 activation/binding domains.

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