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Table 2.

Rescue of enteric neuron development by mRNA injection

Injected mRNA
MorpholinoNonegfra1agfra1bgfra2Control
gfra1a 64.2±2.8 93.1±3.5* 95.6±3.3* 96.6±3.5* 100±4.8 
gfra1a+gfra1b 4.9±0.7 58.5±1.7* N.D. 60.8±3.5* 100±2.5 
ret 0.9±0.4 N.D. 1.6±0.5 0.6±0.4 100±5.7 
Injected mRNA
MorpholinoNonegfra1agfra1bgfra2Control
gfra1a 64.2±2.8 93.1±3.5* 95.6±3.3* 96.6±3.5* 100±4.8 
gfra1a+gfra1b 4.9±0.7 58.5±1.7* N.D. 60.8±3.5* 100±2.5 
ret 0.9±0.4 N.D. 1.6±0.5 0.6±0.4 100±5.7 

Embryos were injected with morpholino oligonucleotides and co-injected with 50 pg of indicated mRNA, fixed and stained with anti-Hu antibody at 96 hpf. Control represents uninjected embryos. Total neurons over a 10-segment length were counted. Numbers represent percent of control±s.e.m. for at least five separate embryos per condition.

N.D., not determined.

*

Significantly different from morpholino injection alone(P<0.0005)

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