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Table 1.

Loss of enteric neurons after morpholino oligonucleotide injection

Morpholino amount (ng)
Morpholino01510
gfra1a 100±6.3 97.6±5.1 63.5±3.9* 60.8±3.8* 
gfra1b 100±6.6 99.5±4.8 98.1±4.7 99.5±4.7 
gfra2 100±6.1 99.2±4.3 100±4.4 96.2±4.3 
ret 100±2.9 92.3±2.1 N.D. 1.8±0.6* 
Morpholino amount (ng)
Morpholino01510
gfra1a 100±6.3 97.6±5.1 63.5±3.9* 60.8±3.8* 
gfra1b 100±6.6 99.5±4.8 98.1±4.7 99.5±4.7 
gfra2 100±6.1 99.2±4.3 100±4.4 96.2±4.3 
ret 100±2.9 92.3±2.1 N.D. 1.8±0.6* 
Morpholino0125
gfra1a+gfra1b 100±4.8 95±3.4 6.9±0.7* 4.7±0.5* 
Morpholino0125
gfra1a+gfra1b 100±4.8 95±3.4 6.9±0.7* 4.7±0.5* 

Embryos were injected with morpholino oligonucleotides, fixed and stained with anti-Hu antibody at 96 hpf. For injection of gfra1a+gfra1b morpholinos, the amount of each oligonucleotide is shown. Total neurons over a 10-segment length were counted. Numbers represent percent of control±s.e.m. for at least five separate embryos per condition. *Significantly different from control(P<0.0005).

N.D., not determined.

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