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Table 2.

Inactivation of Cdc2p-as promotes the late-anaphase configuration of the SIN

Strain Location Percentage
Mob1p-GFP: T0   Medial ring   11  
Mob1p-GFP: T10 DMSO   Medial ring   22  
Mob1p-GFP: T10 analogue   Medial ring   80  
Bgs1p-GFP: T0   Medial ring   18  
Bgs1p-GFP: T10 DMSO   Medial ring   18  
Bgs1p-GFP: T10 analogue   Medial ring   78  
Cdc7p-GFP: T0   2 SPB   83  
Cdc7p-GFP: T10 DMSO   2 SPB   75  
Cdc7p-GFP: T10 analogue   2 SPB   12  
Cdc7p-GFP flp1Δ: T0   2 SPB   79  
Cdc7p-GFP flp1Δ: T10 DMSO   2 SPB   89  
Cdc7p-GFP flp1Δ: T10 analogue   2 SPB   36  
Plo1p-GFP: T0   On SPBs   86  
Plo1p-GFP: T10 DMSO   On SPBs   82  
Plo1p-GFP: T10 analogue   On SPBs   7  
Plo1p-GFP flp1Δ: T0   On SPBs   85  
Plo1p-GFP flp1Δ: T10 DMSO   On SPBs   89  
Plo1p-GFP flp1Δ: T10 analogue   On SPBs   28  
Strain Location Percentage
Mob1p-GFP: T0   Medial ring   11  
Mob1p-GFP: T10 DMSO   Medial ring   22  
Mob1p-GFP: T10 analogue   Medial ring   80  
Bgs1p-GFP: T0   Medial ring   18  
Bgs1p-GFP: T10 DMSO   Medial ring   18  
Bgs1p-GFP: T10 analogue   Medial ring   78  
Cdc7p-GFP: T0   2 SPB   83  
Cdc7p-GFP: T10 DMSO   2 SPB   75  
Cdc7p-GFP: T10 analogue   2 SPB   12  
Cdc7p-GFP flp1Δ: T0   2 SPB   79  
Cdc7p-GFP flp1Δ: T10 DMSO   2 SPB   89  
Cdc7p-GFP flp1Δ: T10 analogue   2 SPB   36  
Plo1p-GFP: T0   On SPBs   86  
Plo1p-GFP: T10 DMSO   On SPBs   82  
Plo1p-GFP: T10 analogue   On SPBs   7  
Plo1p-GFP flp1Δ: T0   On SPBs   85  
Plo1p-GFP flp1Δ: T10 DMSO   On SPBs   89  
Plo1p-GFP flp1Δ: T10 analogue   On SPBs   28  

Cultures of cdc2-as leu1-32 and the markers indicated in the table, were transformed with pREP1 expressing mad2 from the full-strength nmt1 promoter (He et al., 1997). Expression was induced as described in Materials and Methods and synchronous cultures were generated by centrifugal elutriation. Cells were fixed as described in Materials and Methods and indirect immunofluorescence was used to detect GFP and tubulin. The table lists the percentage of cells with the antigen at the location noted in column two, for cells with spindles of ⩽3 μm. The results of a representative experiment are given in each case. 150 to 200 cells with spindles were counted for each sample

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