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Table 2.

Selective translocation of PKC isozymes in osteoclasts by BzATP

Fusion protein % Cells exhibiting translocation Number of cells tested Number of independent transfections
PKCα-EGFP   88   77   14  
PKCβ1-EGFP   79   19   5  
PKCδ-EGFP   10   29   8  
EGFP alone   0   9   3  
Fusion protein % Cells exhibiting translocation Number of cells tested Number of independent transfections
PKCα-EGFP   88   77   14  
PKCβ1-EGFP   79   19   5  
PKCδ-EGFP   10   29   8  
EGFP alone   0   9   3  

Membrane localization of EGFP-tagged PKC isozymes was quantified from fluorescence intensity profiles of confocal images as described in the Results. Osteoclasts were stimulated with BzATP (150 μM) and, after treatment for 0.5-3 minutes, images were analyzed. All isozymes exhibited cytoplasmic localization under unstimulated conditions

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