Selective translocation of PKC isozymes in osteoclasts by BzATP
| Fusion protein . | % Cells exhibiting translocation . | Number of cells tested . | Number of independent transfections . |
|---|---|---|---|
| PKCα-EGFP | 88 | 77 | 14 |
| PKCβ1-EGFP | 79 | 19 | 5 |
| PKCδ-EGFP | 10 | 29 | 8 |
| EGFP alone | 0 | 9 | 3 |
| Fusion protein . | % Cells exhibiting translocation . | Number of cells tested . | Number of independent transfections . |
|---|---|---|---|
| PKCα-EGFP | 88 | 77 | 14 |
| PKCβ1-EGFP | 79 | 19 | 5 |
| PKCδ-EGFP | 10 | 29 | 8 |
| EGFP alone | 0 | 9 | 3 |
Membrane localization of EGFP-tagged PKC isozymes was quantified from fluorescence intensity profiles of confocal images as described in the Results. Osteoclasts were stimulated with BzATP (150 μM) and, after treatment for 0.5-3 minutes, images were analyzed. All isozymes exhibited cytoplasmic localization under unstimulated conditions