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Table 1.

Quantification of microtubule phenotypes

Genotype Value (%) Normalised to wt (%) n
Drosophila primary neurons     
   wt   10.9   100   294  
   shotsf20  35.64   327   195  
   shot3  39.9   366   68  
   shotsf20+ FL 15.65 144  85  
   shot3+ ΔCalp1   56.4   518   35  
   shotkakP2  32.5   298   34  
   shotsf20+ ΔGas2   60   550   57  
   shotsf20+ EGG   50   459   30  
   shot3+ ΔEF 11.1 102  66  
RA-induced mouse N2A cells     
   N2A control   48.4   100   163  
   N2A siRNA1   60.4   125   153  
   N2A siRNA2   65.6   136   116  
Mouse primary cortical neurons     
   Cortical control   24.5   100   105  
   Cortical siRNA1   61.7   252   99  
   Cortical siRNA2   69.0   281   107  
Genotype Value (%) Normalised to wt (%) n
Drosophila primary neurons     
   wt   10.9   100   294  
   shotsf20  35.64   327   195  
   shot3  39.9   366   68  
   shotsf20+ FL 15.65 144  85  
   shot3+ ΔCalp1   56.4   518   35  
   shotkakP2  32.5   298   34  
   shotsf20+ ΔGas2   60   550   57  
   shotsf20+ EGG   50   459   30  
   shot3+ ΔEF 11.1 102  66  
RA-induced mouse N2A cells     
   N2A control   48.4   100   163  
   N2A siRNA1   60.4   125   153  
   N2A siRNA2   65.6   136   116  
Mouse primary cortical neurons     
   Cortical control   24.5   100   105  
   Cortical siRNA1   61.7   252   99  
   Cortical siRNA2   69.0   281   107  

Drosophila primary neurons of different genotypes (as explained in Fig. 5) cultured on glass, retinoic-acid-induced N2A cells on polylysine, and mouse primary cortical neurons on poly-L-ornithine were analysed for non-coalescence of microtubules (compare Figs 1F, 2B,C,E,F,H,J and 3D)

Values (%) and sample numbers (n) are given. Bold face indicates genotypes that display rescue of the mutant phenotype

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