Skip to Main Content
Table 3.

PDE* axon pathfinding defects and ectopic plasma membrane extensions caused by constitutively active rac(G12V) mutant transgenes

GenotypePercent ventral axon guidance errorsPercent ectopic axonsPercent abnormal cellular morphology§
2±3 
ced-10(G12V) (lqEx74) 3±3 100 23±7 
ced-10(+) (lqEx91) 8±6 
mig-2(G16V) (lqEx96) 2±4 25±10 5±4 
mig-2(rh17) 12±5 51±11 2±4 
mig-2(+) (lqEx93) 11±4 
rac-2(G12V) (lqEx48) 1±1 67±6 12±4 
rac-2(+) (lqEx85) 3±2 1±2 
unc-115(ky275) 20±5 
unc-115(ky275); ced-10(G12V) 12±3 100 31±5 
unc-115(ky275); mig-2(G16V) 4±2 52±6 12±4 
unc-115(ky275) mig-2(rh17) 15±4 43±7 4±3 
unc-115(ky275); rac-2(G12V) 26±5 1±1 
GenotypePercent ventral axon guidance errorsPercent ectopic axonsPercent abnormal cellular morphology§
2±3 
ced-10(G12V) (lqEx74) 3±3 100 23±7 
ced-10(+) (lqEx91) 8±6 
mig-2(G16V) (lqEx96) 2±4 25±10 5±4 
mig-2(rh17) 12±5 51±11 2±4 
mig-2(+) (lqEx93) 11±4 
rac-2(G12V) (lqEx48) 1±1 67±6 12±4 
rac-2(+) (lqEx85) 3±2 1±2 
unc-115(ky275) 20±5 
unc-115(ky275); ced-10(G12V) 12±3 100 31±5 
unc-115(ky275); mig-2(G16V) 4±2 52±6 12±4 
unc-115(ky275) mig-2(rh17) 15±4 43±7 4±3 
unc-115(ky275); rac-2(G12V) 26±5 1±1 
*

PDE axon morphology was scored using the integrated transgenelqIs3[osm-6::gfp].

The percent of PDE axons that failed to reach the ventral nerve cord(±2× standard error of the proportion).

The percent of PDE axons that exhibited ectopic axons emanating from the cell body or branching from the main axon (±2× standard error of the proportion).

§

The percentage of PDE neurons exhibiting ectopic morphological defects including plasma membrane sheet-like protrusions and thin extensions(±2× standard error of the proportion).

Close Modal

or Create an Account

Close Modal
Close Modal