Reducing the activity of hlh-2 and hlh-3 causes the NSM sister cells to survive
| A . | . | . | . | ||
|---|---|---|---|---|---|
| . | % NSM sister cell survival (n) at: . | . | . | ||
| Genotype . | 15°C . | 20°C . | 25°C . | ||
| +/+ | 0 (416) | 0 (414) | 0 (408) | ||
| hlh-2(bx108) | 1 (350) | 4 (400) | 5 (410) | ||
| hlh-6(RNAi) | 0 (116) | ||||
| hlh-2(RNAi) | 15 (130) | ||||
| A . | . | . | . | ||
|---|---|---|---|---|---|
| . | % NSM sister cell survival (n) at: . | . | . | ||
| Genotype . | 15°C . | 20°C . | 25°C . | ||
| +/+ | 0 (416) | 0 (414) | 0 (408) | ||
| hlh-2(bx108) | 1 (350) | 4 (400) | 5 (410) | ||
| hlh-6(RNAi) | 0 (116) | ||||
| hlh-2(RNAi) | 15 (130) | ||||
| B . | . |
|---|---|
| Genotype . | % NSM sister cell survival (n) at 25°C . |
| hlh-6(RNAi) | 0±0 (136) |
| hlh-2(bx108) | 5 (410) |
| hlh-2(bx108);hlh-6(RNAi) | 4±1 (176) |
| hlh-3(RNAi) | 7±3 (178) |
| hlh-2(bx108);hlh-3(RNAi) | 30±11 (140) |
| B . | . |
|---|---|
| Genotype . | % NSM sister cell survival (n) at 25°C . |
| hlh-6(RNAi) | 0±0 (136) |
| hlh-2(bx108) | 5 (410) |
| hlh-2(bx108);hlh-6(RNAi) | 4±1 (176) |
| hlh-3(RNAi) | 7±3 (178) |
| hlh-2(bx108);hlh-3(RNAi) | 30±11 (140) |
(A) Reducing the activity of hlh-2 causes NSM sister cell survival. The presence of NSM sister cells was scored as described in the Materials and Methods using the Ptph-1gfp reporter. dsRNA for RNAi was delivered by feeding. The complete genotype of the animals scored was as follows: bcls25, hlh-2(bx108); bcls25, hlh-6(RNAi); bcls25, hlh-2(RNAi); bcls25.
(B) Reducing the activity of hlh-3 causes NSM sister cell survival and enhances hlh-2(bx108). The presence of NSM sister cells was scored as described in the Materials and Methods using the Ptph-1gfp reporter. dsRNA for RNAi was delivered by injections. The complete genotype of the animals scored were as follows: hlh-6(RNAi); bcls25, hlh-2(bx108); bcls25, hlh-2(bx108); hlh-6(RNAi); bcls25, hlh-3(RNAi); bcls25, hlh-2(bx108); hlh-3(RNAi); bcls25. Values are average percent survival obtained for the progeny of different injected animals±s.d.