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Table 1.

Axon guidance defects in zag-1 mutants

GFP markerNeurons labeledProcesses analysedDefect% Animals defectivezag-1 allele
evIs82A (unc-129::GFP) DA + DB motorneurons Axons Ventral cord defasciculation 42 (n=114) rh315 
  Commissures Outgrowth on the wrong side 93 (n=95) rh315 
   Not reaching dorsal cord 84 (n=100) rh315 
   Dorsal cord defasciculation 69 (n=102) rh315 
oxIs12 (unc-47::GFP) DD + VD motorneurons Axon Ventral cord defasciculation 43 (n=100) rh315 
   Gap in ventral cord 0 (n=100) rh315 
  Commissures* Outgrowth on the wrong side 73 (n=100) rh315 
   No outgrowth out of ventral cord 12 (n=100) rh315 
   Not reaching dorsal cord 6 (n=100) rh315 
   Missing branch 17 (n=100) rh315 
   Premature/ectopic branch 30 (n=100) rh315 
oxIs12 (unc-47::GFP) Motorneurons* Axons Ventral cord defasciculation 88 (n=77) hd16 
   Gap in ventral cord 83 (n=78) hd16 
  Commissures Outgrowth on the wrong side 93 (n=107) hd16 
   No outgrowth out of ventral cord 72 (n=76) hd16 
wdIs6 (del-1::GFP) SABVR/L interneurons Axons Axon(s) loop back/change tract 50 (n=104) rh315 
gvEx173 (opt-3::GFP) AVEL/R interneurons Axons Axon(s) extending laterally 16 (n=114) rh315 
rhIs4 (glr-1::GFP) Ventral cord interneurons Axons Ventral cord defasciculation 55 (n=147) rh315 
   Axon extending laterally 97 (n=67) rh315 
hdIs10 (glr-1::GFP) Ventral cord interneurons* Axons Ventral cord defasciculation 70 (n=64) hd16 
   Axon extending laterally 90 (n=64) hd16 
GFP markerNeurons labeledProcesses analysedDefect% Animals defectivezag-1 allele
evIs82A (unc-129::GFP) DA + DB motorneurons Axons Ventral cord defasciculation 42 (n=114) rh315 
  Commissures Outgrowth on the wrong side 93 (n=95) rh315 
   Not reaching dorsal cord 84 (n=100) rh315 
   Dorsal cord defasciculation 69 (n=102) rh315 
oxIs12 (unc-47::GFP) DD + VD motorneurons Axon Ventral cord defasciculation 43 (n=100) rh315 
   Gap in ventral cord 0 (n=100) rh315 
  Commissures* Outgrowth on the wrong side 73 (n=100) rh315 
   No outgrowth out of ventral cord 12 (n=100) rh315 
   Not reaching dorsal cord 6 (n=100) rh315 
   Missing branch 17 (n=100) rh315 
   Premature/ectopic branch 30 (n=100) rh315 
oxIs12 (unc-47::GFP) Motorneurons* Axons Ventral cord defasciculation 88 (n=77) hd16 
   Gap in ventral cord 83 (n=78) hd16 
  Commissures Outgrowth on the wrong side 93 (n=107) hd16 
   No outgrowth out of ventral cord 72 (n=76) hd16 
wdIs6 (del-1::GFP) SABVR/L interneurons Axons Axon(s) loop back/change tract 50 (n=104) rh315 
gvEx173 (opt-3::GFP) AVEL/R interneurons Axons Axon(s) extending laterally 16 (n=114) rh315 
rhIs4 (glr-1::GFP) Ventral cord interneurons Axons Ventral cord defasciculation 55 (n=147) rh315 
   Axon extending laterally 97 (n=67) rh315 
hdIs10 (glr-1::GFP) Ventral cord interneurons* Axons Ventral cord defasciculation 70 (n=64) hd16 
   Axon extending laterally 90 (n=64) hd16 
*

L1 larvae analysed.

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