Yeast strains used in this study
| Strain . | Genotype . | Source . |
|---|---|---|
| Fy2214 | h−leul-32 ura4-D18 ade6-210 GFP-Swi6-LEU2 | Pidoux et al., 2000 |
| Hu334 | h+ade6-210 leul-32 ura4-D18 Pom152-GFP::kanMX6 | This study |
| Hu500 | h−Ndc80-GFP::kanMX6 ura4-Bub1-HA::ura4+ leul-32 ade6 | Wigge and Kilmartin, 2001 |
| Hu607 | rik1::LEU2 Ndc80-GFP::kanMX6 ura4 ade6 | This study |
| Hu633 | leu1-32 mis6-302 ura4-DSE/D18 ade6-210/NCO GFP-Swi6-LEU2 | This study |
| Hu634 | leu1-32 mis6-302 ura4-DSE/D18 ade6-210/NCO Ndc80-GFP::kanMX6 | This study |
| Hu810 | h+Cut12-GFP::ura4+ CFP-Cnp1::kanMX6 leu-132 ura4-DSE/D18 | This study |
| Hu849 | h−Cut12-CFP::kanMX6 Ndc80-GFP::kanMX6 ade6 leu-132 | This study |
| Hu891 | h+leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/dis1 GFP-LacI (HIS7) | This study |
| Hu899 | mis6-302 leu1-32 ura4-DSE/D18 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu900 | mis6-302 ade6-210/NCO leu1-32 ura4-DSE/D18 Pom152-GFP::kanMX6 | This study |
| Hu901 | rik1::LEU2 leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu903 | rikI::LEU2 ade6-210 leu1-32 ura4-DSE/D18 Pom152-GFP::kanMX6 | This study |
| Hu908 | nuf2-1 leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu981 | Cut12-CFP::kanMX6 Ndc80-GFP::kanMX6 CFP-Cnp1::kanMX6 leu1-32 ade6 ura4 | This study |
| Hu1048 | h+/h−diploid Cut12-CFP::kanMX6 Cut12-GFP::ura4+ | This study |
| Strain . | Genotype . | Source . |
|---|---|---|
| Fy2214 | h−leul-32 ura4-D18 ade6-210 GFP-Swi6-LEU2 | Pidoux et al., 2000 |
| Hu334 | h+ade6-210 leul-32 ura4-D18 Pom152-GFP::kanMX6 | This study |
| Hu500 | h−Ndc80-GFP::kanMX6 ura4-Bub1-HA::ura4+ leul-32 ade6 | Wigge and Kilmartin, 2001 |
| Hu607 | rik1::LEU2 Ndc80-GFP::kanMX6 ura4 ade6 | This study |
| Hu633 | leu1-32 mis6-302 ura4-DSE/D18 ade6-210/NCO GFP-Swi6-LEU2 | This study |
| Hu634 | leu1-32 mis6-302 ura4-DSE/D18 ade6-210/NCO Ndc80-GFP::kanMX6 | This study |
| Hu810 | h+Cut12-GFP::ura4+ CFP-Cnp1::kanMX6 leu-132 ura4-DSE/D18 | This study |
| Hu849 | h−Cut12-CFP::kanMX6 Ndc80-GFP::kanMX6 ade6 leu-132 | This study |
| Hu891 | h+leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/dis1 GFP-LacI (HIS7) | This study |
| Hu899 | mis6-302 leu1-32 ura4-DSE/D18 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu900 | mis6-302 ade6-210/NCO leu1-32 ura4-DSE/D18 Pom152-GFP::kanMX6 | This study |
| Hu901 | rik1::LEU2 leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu903 | rikI::LEU2 ade6-210 leu1-32 ura4-DSE/D18 Pom152-GFP::kanMX6 | This study |
| Hu908 | nuf2-1 leu1-32 ura4 Cut12-CFP::kanMX6 lacO (LYS1)/nmt1 GFP-LacI-NLS (HIS7) | This study |
| Hu981 | Cut12-CFP::kanMX6 Ndc80-GFP::kanMX6 CFP-Cnp1::kanMX6 leu1-32 ade6 ura4 | This study |
| Hu1048 | h+/h−diploid Cut12-CFP::kanMX6 Cut12-GFP::ura4+ | This study |