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Table 3.

Cell-cycle-dependent and oriP specific x-fold enrichment of HsMcm3p and HsMcm7p

G1 G1/S S S/G2 G2/M
A. MCM3      
sc2   1.84   2.3   0.6   1.5   0.9  
sc3   1.9   2.2   0.9   1.5   1.9  
sc4   10.0   9.4   1.3   4.0   1.7  
sc5   23.9   9.5   1.7   4.5   4.4  
sc6   19.0   13.9   2.5   5.4   4.2  
sc7   15.6   12.0   1.8   2.6   1.4  
sc8   16.7   11.2   1.4   3.5   2.4  
sc10   0.8   0.6   0.4   0.5   0.4  
B. MCM7      
sc2   1.3   0.9   0.6   0.7   1.0  
sc3   0.8   2.1   0.7   1.0   0.8  
sc4   6.9   7.3   1.5   2.2   1.9  
sc5   15.2   12.7   1.9   3.1   3.6  
sc6   10.4   8.3   2.0   2.9   2.9  
sc7   7.6   6.5   1.2   1.4   1.0  
sc8   10.8   11.0   1.5   5.3   1.5  
sc10   0.8   0.5   0.6   0.5   0.6  
G1 G1/S S S/G2 G2/M
A. MCM3      
sc2   1.84   2.3   0.6   1.5   0.9  
sc3   1.9   2.2   0.9   1.5   1.9  
sc4   10.0   9.4   1.3   4.0   1.7  
sc5   23.9   9.5   1.7   4.5   4.4  
sc6   19.0   13.9   2.5   5.4   4.2  
sc7   15.6   12.0   1.8   2.6   1.4  
sc8   16.7   11.2   1.4   3.5   2.4  
sc10   0.8   0.6   0.4   0.5   0.4  
B. MCM7      
sc2   1.3   0.9   0.6   0.7   1.0  
sc3   0.8   2.1   0.7   1.0   0.8  
sc4   6.9   7.3   1.5   2.2   1.9  
sc5   15.2   12.7   1.9   3.1   3.6  
sc6   10.4   8.3   2.0   2.9   2.9  
sc7   7.6   6.5   1.2   1.4   1.0  
sc8   10.8   11.0   1.5   5.3   1.5  
sc10   0.8   0.5   0.6   0.5   0.6  

To verify the oriP-specific enrichment of HsMcm3p-HsMcm7p, Econst was determined from standard curves of tenfold dilutions as described (Schepers et al., 2001). The Cp differences and standard deviations of three independent experiments are shown for the five fractions analysed. The mean values of each DNA segment and cell-cycle phase were determined (Cp difference). Because EBNA1 is cell-cycle-independently bound, the mean value of each fragment was established (Cp average) and used as exponent to determine the enrichment by the equation N=EconstΔCp (average enrichment). This table summarizes the data of the histograms in Fig. 4.

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