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Table 2.

Enrichment of HsOrc3p, HsOrc6p and HsOrc1p at oriP during the cell cycle

x-fold enrichment above reference level (I3-fragment)
Primer G1 G1/S S S/G2 G2/M Average
A. HsOrc3p       
sc2   0.9   1.6   1.9   2.4   2.0   1.8±0.5  
sc3   1.3   1.6   2.0   1.9   2.0   1.8±0.3  
sc4   8.5   7.9   10.2   10.2   11.1   9.6±1.3  
sc5   18.3   20.1   24.1   21.5   26.9   22.2±3.4  
sc6   22.7   14.7   20.3   19.4   18.1   19.0±2.9  
sc7   11.7   11.5   12.5   14.3   13.1   12.6±1.1  
sc8   6.3   6.6   8.7   9.7   10.0   8.3±1.7  
sc10   0.7   1.1   1.0   1.3   0.9   1.0±0.2  
B. HsOrc6p       
sc2   0.9   1.2   1.2   1.4   1.0   1.1±0.2  
sc3   0.8   0.9   1.1   0.9   1.0   1.0±0.1  
sc4   2.6   2.6   2.1   2.3   2.1   2.3±0.3  
sc5   3.5   3.2   2.7   3.5   3.2   3.2±0.3  
sc6   2.8   2.3   2.2   3.2   3.0   2.7±0.5  
sc7   2.8   3.0   2.6   2.8   3.2   2.9±0.2  
sc8   2.5   2.5   2.7   2.4   2.5   2.5±0.1  
sc10   1.3   1.3   1.3   1.7   1.6   1.4±0.2  
C. HsOrc1       
sc2   1.1   1.3   1.2   1.3   1.2   
sc3   0.9   1.4   1.6   1.3   1.2   
sc4   9.8   12.9   5.9   6.4   6.2   
sc5   23.4   24.3   9.2   6.1   9.4   
sc6   21.8   27.4   9.1   9.2   8.9   
sc7   16.6   15.9   7.9   5.8   7.2   
sc8   6.9   7.6   4.2   4.3   5.3   
sc10   1.9   1.8   1.4   1.3   1.4   
x-fold enrichment above reference level (I3-fragment)
Primer G1 G1/S S S/G2 G2/M Average
A. HsOrc3p       
sc2   0.9   1.6   1.9   2.4   2.0   1.8±0.5  
sc3   1.3   1.6   2.0   1.9   2.0   1.8±0.3  
sc4   8.5   7.9   10.2   10.2   11.1   9.6±1.3  
sc5   18.3   20.1   24.1   21.5   26.9   22.2±3.4  
sc6   22.7   14.7   20.3   19.4   18.1   19.0±2.9  
sc7   11.7   11.5   12.5   14.3   13.1   12.6±1.1  
sc8   6.3   6.6   8.7   9.7   10.0   8.3±1.7  
sc10   0.7   1.1   1.0   1.3   0.9   1.0±0.2  
B. HsOrc6p       
sc2   0.9   1.2   1.2   1.4   1.0   1.1±0.2  
sc3   0.8   0.9   1.1   0.9   1.0   1.0±0.1  
sc4   2.6   2.6   2.1   2.3   2.1   2.3±0.3  
sc5   3.5   3.2   2.7   3.5   3.2   3.2±0.3  
sc6   2.8   2.3   2.2   3.2   3.0   2.7±0.5  
sc7   2.8   3.0   2.6   2.8   3.2   2.9±0.2  
sc8   2.5   2.5   2.7   2.4   2.5   2.5±0.1  
sc10   1.3   1.3   1.3   1.7   1.6   1.4±0.2  
C. HsOrc1       
sc2   1.1   1.3   1.2   1.3   1.2   
sc3   0.9   1.4   1.6   1.3   1.2   
sc4   9.8   12.9   5.9   6.4   6.2   
sc5   23.4   24.3   9.2   6.1   9.4   
sc6   21.8   27.4   9.1   9.2   8.9   
sc7   16.6   15.9   7.9   5.8   7.2   
sc8   6.9   7.6   4.2   4.3   5.3   
sc10   1.9   1.8   1.4   1.3   1.4   

Table 2 summarizes the oriP-specific enrichment shown in the histograms in Fig. 3. Econst was determined from standard curves of tenfold dilutions as described (Schepers et al., 2001). The Cp differences and standard deviations of three independent experiments are shown for the five fractions analysed. The mean values of each DNA segment and cell-cycle phase were determined (Cp difference). Because EBNA1 is cell-cycle-independently bound, the mean value of each fragment was established (Cp average) and used as exponent to determine the enrichment by the equation N=EconstΔCp (average enrichment). The mean value of the reference fragment I3 was determined and used as divisor to calculate the accumulation for the scanning fragments. The obtained numbers represent the enrichment at the particular fragment above the I3 level.

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