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Table 2.

Behavior of zebrafish LAP2-GFP fusion proteins in transfected Xenopus A6 cells during mitosis

ZLAP2-GFP fusion proteinAssociation with mitotic chromosomes
1-214GFP (common domain) +/− 
1-360GFP (β) +++ 
1-502GFP (ω) +++ 
214-314GFP (ω)  
315-460GFP(ω)*  
214-502GFP (ω)  
ZLAP2-GFP fusion proteinAssociation with mitotic chromosomes
1-214GFP (common domain) +/− 
1-360GFP (β) +++ 
1-502GFP (ω) +++ 
214-314GFP (ω)  
315-460GFP(ω)*  
214-502GFP (ω)  
*

Identical to amino acids 215-360 of zebrafish LAP2β.

Numbers denote amino acids of the zebrafish LAP2 isoforms listed in brackets (see also Fig. 1A). In the fusion proteins used GFP was always localized at the C-terminus of ZLAP2.

+/−

, weak staining of chromosomes.

+++

, intense staining of chromosomes.

, no chromosome staining.

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