Degree of response to all stimuli
| . | Response . | . | ||
|---|---|---|---|---|
| Stimulus . | SGK (wt) . | **SGK (S/D) . | ||
| HGF | 2.2 (±0.2) n=9 | 1.5 (±0.1) n=3 | ||
| Rac V12 | 2.2 (±0.2) n=10 | 1.6 (±0.7) n=2 | ||
| Fibronectin | 3.7 (±0.5) n=8 | 2.5 (±0.6) n=3 | ||
| Table 1 represents the degree of response to all stimuli. SGK (S/D) has an intrinsic activity that is 2.4(±0.4) n=7 fold greater than SGK (wt) in the absence of stimuli. There was no activity detected in SGK mutants T256A, S422A or T256A/S422A using HGF, Rac1-V12 or fibronectin. | ||||
| . | Response . | . | ||
|---|---|---|---|---|
| Stimulus . | SGK (wt) . | **SGK (S/D) . | ||
| HGF | 2.2 (±0.2) n=9 | 1.5 (±0.1) n=3 | ||
| Rac V12 | 2.2 (±0.2) n=10 | 1.6 (±0.7) n=2 | ||
| Fibronectin | 3.7 (±0.5) n=8 | 2.5 (±0.6) n=3 | ||
| Table 1 represents the degree of response to all stimuli. SGK (S/D) has an intrinsic activity that is 2.4(±0.4) n=7 fold greater than SGK (wt) in the absence of stimuli. There was no activity detected in SGK mutants T256A, S422A or T256A/S422A using HGF, Rac1-V12 or fibronectin. | ||||
SGK (SD) has the point mutation S422D.