Two-hybrid analysis of interaction between DmUba2 and DmAos1a
| . | Fusion plasmid . | . | |
|---|---|---|---|
| Fusion plasmidb . | DBD-DmAos1 . | AD-DmAos1 . | |
| DmUba2-F | 908 | 143c | |
| DmUba2-N | 52 | 15 | |
| DmUba2-C | 57 | 35 | |
| Anillin | 40 | 27 | |
| No fusion | 43 | 25 | |
| . | Fusion plasmid . | . | |
|---|---|---|---|
| Fusion plasmidb . | DBD-DmAos1 . | AD-DmAos1 . | |
| DmUba2-F | 908 | 143c | |
| DmUba2-N | 52 | 15 | |
| DmUba2-C | 57 | 35 | |
| Anillin | 40 | 27 | |
| No fusion | 43 | 25 | |
Two-hybrid tests between the indicated pairs of plasmids were performed as described in Materials and Methods. Each entry shows the units ofβ-galactosidase activity.
The DBD or AD fusion, as appropriate. DmUba2-F plasmids contain full-length DmUba2; DmUba2-N and DmUba2-C plasmids contain the N-terminus (amino acids 1-350) and C-terminus (amino acids 351-700) of DmUba2, respectively (see Materials and Methods).
The empty vector pJG4-5PL and the full-length AD-anillin clone (see Materials and Methods) were used as negative controls with DBD-DmUba2-F; each gave ∼40 units of β-galactosidase activity.