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Table 2.

Regulation of dauer formation and germline proliferation

Genotype% Dauer arrest (n)Number of germ cell nuclei in dauer*(n)
(A) N2 0 (124) 39.92±4.58(25)§ 
(B) aak-2(ok524) 0 (148) 60.36±9.30A(25)§ 
(C) daf-2(e1370) 100 (293) 35.44±3.85 (25) 
(D) daf-2(e1370); aak-2(rr48) 100 (156) 96.16±18.06C(25) 
(E) daf-2(e1370); aak-2(ok524) 100 (290) 69.96±9.08C(25) 
(F) daf-2(e1370); aak-1(RNAi) 100 (95) 47.77±9.26C(30) 
(G) daf-2(e1370); daf-18(e1375) 99.48 (763) 98.17±13.61C(30) 
(H) daf-2(e1370); akt-1(mg144)gf 97.7 (607) 50.82±6.57C(28) 
(I) daf-2(e1370); akt-1(mg144)gf; aak-2(rr48) 77.4 (1387) 126.60±18.95D(15) 
(J) daf-2(e1370); aak-2(rr48); aak-1(RNAi) ND 165.00±36.13D(28) 
(K) daf-2(e1370); akt-1(mg144)gf; aak-2(ok524) 67.7 (653) 85.33±12.40E(15) 
(L) daf-2(e1370); aak-2(ok524); aak-1(RNAi) ND 152.64±32.51E(25) 
(M) daf-2(e1370); daf-18(e1375); aak-2(ok524); aak-1(RNAi) 85.13 (316) 189.53±23.71L(15) 
(N) daf-7(e1372) 100 (182) 33.19±4.07 (26) 
(O) daf-7(e1372); aak-2(rr48) 100 (201) 80.12±6.44N(25) 
(P) daf-7(e1372); aak-2(ok524) 100 (153) 65.24±14.85N(25) 
(Q) daf-7(e1372); aak-1(RNAi) 100 (311) 44.60±10.68N(25) 
(R) daf-7(e1372); daf-18(e1375) 99.8 (577) 87.24±14.41N(25) 
(S) daf-7(e1372); daf-18(ok480) 92.8 (376) 177.48±21.48N(25) 
(T) daf-7(e1372); akt-1(mg144)gf 100 (423) 32.15±2.60N(26) 
(U) daf-16(mgDf50); daf-7(e1372) 98.7 (234) 39.68±4.89N(25) 
(V) daf-16(mgDf50); daf-7(e1372); aak-2(rr48) 32.0 (222) 95.24±10.65U(25) 
(W) daf-16(mgDf50); daf-7(e1372); aak-2(ok524) 50.5 (497) 82.93±10.14U(15) 
Genotype% Dauer arrest (n)Number of germ cell nuclei in dauer*(n)
(A) N2 0 (124) 39.92±4.58(25)§ 
(B) aak-2(ok524) 0 (148) 60.36±9.30A(25)§ 
(C) daf-2(e1370) 100 (293) 35.44±3.85 (25) 
(D) daf-2(e1370); aak-2(rr48) 100 (156) 96.16±18.06C(25) 
(E) daf-2(e1370); aak-2(ok524) 100 (290) 69.96±9.08C(25) 
(F) daf-2(e1370); aak-1(RNAi) 100 (95) 47.77±9.26C(30) 
(G) daf-2(e1370); daf-18(e1375) 99.48 (763) 98.17±13.61C(30) 
(H) daf-2(e1370); akt-1(mg144)gf 97.7 (607) 50.82±6.57C(28) 
(I) daf-2(e1370); akt-1(mg144)gf; aak-2(rr48) 77.4 (1387) 126.60±18.95D(15) 
(J) daf-2(e1370); aak-2(rr48); aak-1(RNAi) ND 165.00±36.13D(28) 
(K) daf-2(e1370); akt-1(mg144)gf; aak-2(ok524) 67.7 (653) 85.33±12.40E(15) 
(L) daf-2(e1370); aak-2(ok524); aak-1(RNAi) ND 152.64±32.51E(25) 
(M) daf-2(e1370); daf-18(e1375); aak-2(ok524); aak-1(RNAi) 85.13 (316) 189.53±23.71L(15) 
(N) daf-7(e1372) 100 (182) 33.19±4.07 (26) 
(O) daf-7(e1372); aak-2(rr48) 100 (201) 80.12±6.44N(25) 
(P) daf-7(e1372); aak-2(ok524) 100 (153) 65.24±14.85N(25) 
(Q) daf-7(e1372); aak-1(RNAi) 100 (311) 44.60±10.68N(25) 
(R) daf-7(e1372); daf-18(e1375) 99.8 (577) 87.24±14.41N(25) 
(S) daf-7(e1372); daf-18(ok480) 92.8 (376) 177.48±21.48N(25) 
(T) daf-7(e1372); akt-1(mg144)gf 100 (423) 32.15±2.60N(26) 
(U) daf-16(mgDf50); daf-7(e1372) 98.7 (234) 39.68±4.89N(25) 
(V) daf-16(mgDf50); daf-7(e1372); aak-2(rr48) 32.0 (222) 95.24±10.65U(25) 
(W) daf-16(mgDf50); daf-7(e1372); aak-2(ok524) 50.5 (497) 82.93±10.14U(15) 

n, sample size.

ND, not determined.

*

, Mean values±s.d.

, Statistical significance using the one-tailed t-test with unequal variance (P≤0.0005), versus row `X'.

, As above but, P=0.14.

§

, Dauer formation (>90% daf-c) was induced using dauer pheromone preparation.

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