PCR primers used in this study
| Primer . | . | Forward . | Reverse . | Positions* . |
|---|---|---|---|---|
| Hoxb8 proximal | 1 | aacaggagacagagaaactggtac | actgtttgctttgctgctgtttag | 1-516 |
| 2 | gggtataaatttctgaaggttaag | agggatgagaagggccgaggg | 446-1006 | |
| 3 | tatgactacctcgttgtttg | caaagactgatgtgggggagt | 4292-4565 | |
| 4 | ggtgtttttccgtgactcccccac | tagaacagcgaagcctgcaaaagt | 4531-5091 | |
| 5 | gccgcggctgccatgcaggcttag | cacggcgcacgggttctgctggta | 5045-5568 | |
| 6 | ttctacggctacgaccctctgcag | cttgagggcgcatccaggg | 5593-5764 | |
| 7 | ccctggatgcgccctcaag | tctccacagcccccataaaac | 5746-6083 | |
| 8 | actgttttatgggggctgtggaga | tctctggtaactagaaccag | 6060-6315 | |
| 9 | tggagctggagaaggagttccta | cagaagctattacgagatactacc | 6592-7041 | |
| 10 | ctccttcccttctcttgggggtcc | caatgctcacagcgcgcatgc | 7061-7529 | |
| 11 | ttagcatgcgcgctgtgagcattg | cactagccaccagcctgggta | 7560-8032 | |
| 12 | cgctcttgggaagagatctaccca | cccaagaggaggcgagcctgg | 7994-8584 | |
| 13 | aggccaggctcgcctcctcttggg | agtcggggacgttttagtgtc | 8561-9085 | |
| 14 | tcacgtggtcagaagagg | ctgagcttcgcatccaggggta | 8978-9505 | |
| 15 | tacccctggatgcgaagctcag | ctagggcctgagagcactgagc | 9484-9706 | |
| 16 | gctcagtgctctcaggccctag | acacccagaactgagctctc | 9685-9927 | |
| 17 | gagagctcagttctgggtgt | accactctttgactctgtgt | 9908-10134 | |
| 18 | acacagagtcaaagagtggt | gtcatctttctggagtgata | 10115-10323 | |
| 19 | tatcactccagaaagatgac | atgagtataggagtctct | 10304-10527 | |
| 20 | agagactcctatactcat | tctgagaactcccagcata | 10501-10792 | |
| 21 | tatgctgggagttctcaga | ctgacagaacttggctctgatg | 10774-10985 | |
| 22 | catcagagccaagttctgtcag | ctgtcatcagtcactctct | 10964-11218 | |
| 23 | agagagtgactgatgacag | agccatcctcctgattcag | 11220-11408 | |
| 24 | ctgcacctagtagtg | tgtaggtctggcggcctcgcttt | 11581-11787 | |
| 25 | ttgtaagccctctttgaagct | taacataaccctcctggcaggccg | 12309-12829 | |
| Hoxb8 distal | D1 | ggtagtagctttctgatggt | aggatgcaaactccattata | |
| D2 | accatcagaaagctactacc | aacgaatttattgagaattc | ||
| Hoxb3 | atgcagaaagccacctacta | ttggacgtttgcctcgactc | ||
| Hoxb6 | atgagttcctatttcgtgaa | accagccggcggcggctacg | ||
| Hoxb8 | atgagctcttatttcgtcaa | gcttgcagtctgcgtactgc | ||
| Hoxb9 | atgtccatttctgggacgct | tggtagacagacggcaggct | ||
| Hoxa4 | agctccagccctggcttcgc | cgtgatggatgcygctagcc | ||
| Adam34 | atgagtgggactaaggccctg | gcggttatgatctattactac |
| Primer . | . | Forward . | Reverse . | Positions* . |
|---|---|---|---|---|
| Hoxb8 proximal | 1 | aacaggagacagagaaactggtac | actgtttgctttgctgctgtttag | 1-516 |
| 2 | gggtataaatttctgaaggttaag | agggatgagaagggccgaggg | 446-1006 | |
| 3 | tatgactacctcgttgtttg | caaagactgatgtgggggagt | 4292-4565 | |
| 4 | ggtgtttttccgtgactcccccac | tagaacagcgaagcctgcaaaagt | 4531-5091 | |
| 5 | gccgcggctgccatgcaggcttag | cacggcgcacgggttctgctggta | 5045-5568 | |
| 6 | ttctacggctacgaccctctgcag | cttgagggcgcatccaggg | 5593-5764 | |
| 7 | ccctggatgcgccctcaag | tctccacagcccccataaaac | 5746-6083 | |
| 8 | actgttttatgggggctgtggaga | tctctggtaactagaaccag | 6060-6315 | |
| 9 | tggagctggagaaggagttccta | cagaagctattacgagatactacc | 6592-7041 | |
| 10 | ctccttcccttctcttgggggtcc | caatgctcacagcgcgcatgc | 7061-7529 | |
| 11 | ttagcatgcgcgctgtgagcattg | cactagccaccagcctgggta | 7560-8032 | |
| 12 | cgctcttgggaagagatctaccca | cccaagaggaggcgagcctgg | 7994-8584 | |
| 13 | aggccaggctcgcctcctcttggg | agtcggggacgttttagtgtc | 8561-9085 | |
| 14 | tcacgtggtcagaagagg | ctgagcttcgcatccaggggta | 8978-9505 | |
| 15 | tacccctggatgcgaagctcag | ctagggcctgagagcactgagc | 9484-9706 | |
| 16 | gctcagtgctctcaggccctag | acacccagaactgagctctc | 9685-9927 | |
| 17 | gagagctcagttctgggtgt | accactctttgactctgtgt | 9908-10134 | |
| 18 | acacagagtcaaagagtggt | gtcatctttctggagtgata | 10115-10323 | |
| 19 | tatcactccagaaagatgac | atgagtataggagtctct | 10304-10527 | |
| 20 | agagactcctatactcat | tctgagaactcccagcata | 10501-10792 | |
| 21 | tatgctgggagttctcaga | ctgacagaacttggctctgatg | 10774-10985 | |
| 22 | catcagagccaagttctgtcag | ctgtcatcagtcactctct | 10964-11218 | |
| 23 | agagagtgactgatgacag | agccatcctcctgattcag | 11220-11408 | |
| 24 | ctgcacctagtagtg | tgtaggtctggcggcctcgcttt | 11581-11787 | |
| 25 | ttgtaagccctctttgaagct | taacataaccctcctggcaggccg | 12309-12829 | |
| Hoxb8 distal | D1 | ggtagtagctttctgatggt | aggatgcaaactccattata | |
| D2 | accatcagaaagctactacc | aacgaatttattgagaattc | ||
| Hoxb3 | atgcagaaagccacctacta | ttggacgtttgcctcgactc | ||
| Hoxb6 | atgagttcctatttcgtgaa | accagccggcggcggctacg | ||
| Hoxb8 | atgagctcttatttcgtcaa | gcttgcagtctgcgtactgc | ||
| Hoxb9 | atgtccatttctgggacgct | tggtagacagacggcaggct | ||
| Hoxa4 | agctccagccctggcttcgc | cgtgatggatgcygctagcc | ||
| Adam34 | atgagtgggactaaggccctg | gcggttatgatctattactac |
Position of 5′-most nucleotide of region 1 was arbitrarily designated as `1'