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Table 2.

Summary of eye development in pax7/pax7EnR injected embryos

ConstructsTwo normal eyes and one extra eyeTwo normal eyesOne small eye and one normal eyeSingle eye
pax7EnR 43 (48.8%) 32 (36.4%) 13 (14.8%) 
pax7EnR+pax7 37 (94.9%) 2 (5.1%) 
pax7 15 (10.0%) 135 (90.0%) 
ConstructsTwo normal eyes and one extra eyeTwo normal eyesOne small eye and one normal eyeSingle eye
pax7EnR 43 (48.8%) 32 (36.4%) 13 (14.8%) 
pax7EnR+pax7 37 (94.9%) 2 (5.1%) 
pax7 15 (10.0%) 135 (90.0%) 

pax7EnR (200 pg) or 500 pg pax7 capped RNA, or both, were injected into one of the dorsal animal hemispheres of four-cell stage embryos. Eye development was measured at stage 46

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