Summary of eye development in pax7/pax7EnR injected embryos
| Constructs . | Two normal eyes and one extra eye . | Two normal eyes . | One small eye and one normal eye . | Single eye . |
|---|---|---|---|---|
| pax7EnR | 0 | 43 (48.8%) | 32 (36.4%) | 13 (14.8%) |
| pax7EnR+pax7 | 0 | 37 (94.9%) | 2 (5.1%) | 0 |
| pax7 | 15 (10.0%) | 135 (90.0%) | 0 | 0 |
| Constructs . | Two normal eyes and one extra eye . | Two normal eyes . | One small eye and one normal eye . | Single eye . |
|---|---|---|---|---|
| pax7EnR | 0 | 43 (48.8%) | 32 (36.4%) | 13 (14.8%) |
| pax7EnR+pax7 | 0 | 37 (94.9%) | 2 (5.1%) | 0 |
| pax7 | 15 (10.0%) | 135 (90.0%) | 0 | 0 |
pax7EnR (200 pg) or 500 pg pax7 capped RNA, or both, were injected into one of the dorsal animal hemispheres of four-cell stage embryos. Eye development was measured at stage 46