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Table 3.

HSCs are highly enriched within the VE-cadherin-expressing populations

Embryo age	Organ	Cells per organ(1×10⁵)^{^*}	HSCs per organ^{^†}	HSC candidate phenotype	Estimated HSC frequency
11.5	AGM	1.4±0.12	1	VE-cadherin⁺CD45⁺	∼1/70
				VE-cadherin^–CD45⁺	–
12.5	YS	2.1±0.2	2	VE-cadherin⁺CD45⁺	∼1/64
				VE-cadherin^–CD45^+/–	–
	PB	28.7±2.9	3	VE-cadherin⁺(CD45⁺)	∼1/48
				VE-cadherin^–(CD45^+/–)	–
13.5	FL	63.6±2.5	260	VE-cadherin⁺CD45⁺	∼1/127
				VE-cadherin⁺CD45⁺	∼1/2,226

Embryo age	Organ	Cells per organ(1×10⁵)^{^*}	HSCs per organ^{^†}	HSC candidate phenotype	Estimated HSC frequency
11.5	AGM	1.4±0.12	1	VE-cadherin⁺CD45⁺	∼1/70
				VE-cadherin^–CD45⁺	–
12.5	YS	2.1±0.2	2	VE-cadherin⁺CD45⁺	∼1/64
				VE-cadherin^–CD45^+/–	–
	PB	28.7±2.9	3	VE-cadherin⁺(CD45⁺)	∼1/48
				VE-cadherin^–(CD45^+/–)	–
13.5	FL	63.6±2.5	260	VE-cadherin⁺CD45⁺	∼1/127
				VE-cadherin⁺CD45⁺	∼1/2,226

Dead cells excluded according to 7-AAD uptake

Previously determined by limiting dilution analysis in unsorted organs(Kumaravelu et al., 2002)