Statistical analysis of VE-cadherin and CD45 expression in major E11.5-E13.5 haematopoietic organs during key stages of HSC development
| . | . | . | Percent phenotype frequency ± s.e.m. (range) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Embryo age . | Organ . | n . | VE-cadherin+CD45– . | VE-cadherin–CD45+ . | VE-cadherin+CD45+ . | VE-cadherin–CD45– . | |||
| 11.5 | AGM | 7 | 2.5±0.4 (1.3-4.0) | 2.6±0.4 (1.6-4.4) | 0.05±0.01 (0.01-0.1) | 95±0.8 (91-96) | |||
| Yolk sac | 4 | 6.9±1.3 (3.5-9.6) | 2.4±0.5 (1.3-3.4) | 0.05±0.02 (0.01-0.08) | 91±1.6 (87-95) | ||||
| Liver | 6 | 10±0.07 (8.9-12.0) | 4.1±0.7 (2.9-6.3) | 1.2±0.2 (0.5-2.1) | 84±1.3 (80-87) | ||||
| 12.5 | AGM | 4 | 2.1±0.2 (1.4-2.4) | 1.5±0.3 (0.9-2.1) | 0.02±0.01 (0.01-0.04) | 96±0.4 (96-98) | |||
| Yolk sac | 3 | 5.3±2.4 (2.4-10.0) | 1.3±0.3 (0.7-1.9) | 0.03±0.01 (0.02-0.04) | 93±2.2 (89-96) | ||||
| PB | 6 | 1.2±0.6 (0.2-4.0) | 0.035±0.005 (0.017-0.045) | 0.005±0.002 (0.001-0.008) | 99±0.6 (96-100) | ||||
| Liver | 5 | 5.5±0.3 (4.5-6.5) | 1.3±0.2 (0.8-1.7) | 0.31±0.04 (0.22-0.42) | 93±0.4 (92-94) | ||||
| 13.5 | Liver | 5 | 3.5±0.4 (2.2-4.4) | 1.2±0.4 (0.5-2.8) | 0.1±0.04 (0.02-0.22) | 95±0.13 (95-96) | |||
| . | . | . | Percent phenotype frequency ± s.e.m. (range) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Embryo age . | Organ . | n . | VE-cadherin+CD45– . | VE-cadherin–CD45+ . | VE-cadherin+CD45+ . | VE-cadherin–CD45– . | |||
| 11.5 | AGM | 7 | 2.5±0.4 (1.3-4.0) | 2.6±0.4 (1.6-4.4) | 0.05±0.01 (0.01-0.1) | 95±0.8 (91-96) | |||
| Yolk sac | 4 | 6.9±1.3 (3.5-9.6) | 2.4±0.5 (1.3-3.4) | 0.05±0.02 (0.01-0.08) | 91±1.6 (87-95) | ||||
| Liver | 6 | 10±0.07 (8.9-12.0) | 4.1±0.7 (2.9-6.3) | 1.2±0.2 (0.5-2.1) | 84±1.3 (80-87) | ||||
| 12.5 | AGM | 4 | 2.1±0.2 (1.4-2.4) | 1.5±0.3 (0.9-2.1) | 0.02±0.01 (0.01-0.04) | 96±0.4 (96-98) | |||
| Yolk sac | 3 | 5.3±2.4 (2.4-10.0) | 1.3±0.3 (0.7-1.9) | 0.03±0.01 (0.02-0.04) | 93±2.2 (89-96) | ||||
| PB | 6 | 1.2±0.6 (0.2-4.0) | 0.035±0.005 (0.017-0.045) | 0.005±0.002 (0.001-0.008) | 99±0.6 (96-100) | ||||
| Liver | 5 | 5.5±0.3 (4.5-6.5) | 1.3±0.2 (0.8-1.7) | 0.31±0.04 (0.22-0.42) | 93±0.4 (92-94) | ||||
| 13.5 | Liver | 5 | 3.5±0.4 (2.2-4.4) | 1.2±0.4 (0.5-2.8) | 0.1±0.04 (0.02-0.22) | 95±0.13 (95-96) | |||