The Company of Biologists

Table 3.

Fgf3 is sufficient to induce phox2a-positive, epibranchial neurons in wild-type and cas embryos

	n^*	Embryos with ectopic phox2a	Ectopic phox2afoci^{^†}	Colocalized with GFP	II^{^‡}	III and IV	Posterior arches
HS-gfp → wt	61	1	1	0	1	0	0
HS-gfp → cas	74	1	1	0	1	0	0
HS-fgf3 →wt^{^§}	72	15	28	20 (71%)	16	3	9
HS-fgf3 → cas^{^§}	78	21	28	20 (71%)	21	0	7

	n^*	Embryos with ectopic phox2a	Ectopic phox2afoci^{^†}	Colocalized with GFP	II^{^‡}	III and IV	Posterior arches
HS-gfp → wt	61	1	1	0	1	0	0
HS-gfp → cas	74	1	1	0	1	0	0
HS-fgf3 →wt^{^§}	72	15	28	20 (71%)	16	3	9
HS-fgf3 → cas^{^§}	78	21	28	20 (71%)	21	0	7

Number of animals assessed in each treatment group.

A number of embryos had more than one ectopic phox2a-positive foci.

II-IV — bronchial arches.

\(P{\ll}0.01\)

(χ² test) when compared with HS-gfp injections.