Neurogenesis in epibranchial placodes requires endoderm and Fgf3*
| . | n† . | Reduced f . | Absent g . | Absent small v . | Reduced large v . |
|---|---|---|---|---|---|
| cas-MO | 36 | 36‡(100%) | 36 (100%) | 36 (100%) | 36‡(100%) |
| SU5402 treatment | 60 | 60§ (100%) | 60 (100%) | 60 (100%) | 60§ (100%) |
| fgf3-MO1 | 45 | 45¶ (100%) | 45 (100%) | 45 (45%) | 60¶ (100%) |
| fgf3-MO2 | 48 | 25 (56%) | 12 (25%) | 14 (29%) | 33 (69%) |
| . | n† . | Reduced f . | Absent g . | Absent small v . | Reduced large v . |
|---|---|---|---|---|---|
| cas-MO | 36 | 36‡(100%) | 36 (100%) | 36 (100%) | 36‡(100%) |
| SU5402 treatment | 60 | 60§ (100%) | 60 (100%) | 60 (100%) | 60§ (100%) |
| fgf3-MO1 | 45 | 45¶ (100%) | 45 (100%) | 45 (45%) | 60¶ (100%) |
| fgf3-MO2 | 48 | 25 (56%) | 12 (25%) | 14 (29%) | 33 (69%) |
wt, wild type; f, facial ganglion; g, glossopharyngeal ganglion; v, vagal ganglion.
All phenotypes assessed by phox2a/phox2b expression at 36 hpf.
Number of embryos in each treatment group.
Significantly reduced compared with to wild type, see Fig. 1A.
Significantly reduced compared with wild type, see Fig. 2A.
Significantly reduced compared with wild type, see Fig. 3A.