Primers used for quantitative real-time PCR
| Gene . | Primer (5′–3′) . | PCR product size (bp) . |
|---|---|---|
| CS | Forward: CCATCGACTCCAACCTGGAC | 51 |
| Reverse: ATCCCAGCATGTTGCTGAAGT | ||
| EF-1A | Forward: CTGGAAGCCAGTGAAAAGATGAC | 51 |
| Reverse: ACGCTCAACCTTCCATCCC | ||
| 18S | Forward: ACCACATCCAAGGAAGGCAG | 51 |
| Reverse: CCGAGTCGGGAGTGGGTAAT | ||
| ND2 | Forward: TAATAGCACAAAATCACCACC | 91 |
| Reverse: GCAAAAAGTAGTACGGCG | ||
| NRF-1 | Forward: TCCAGCAGGTTCAGGTACATGT | 51 |
| Reverse: TCATCATGGACGCTTCGGTAT | ||
| PGC-1α | Forward: CTCCAAATGACCACAAGGGATC | 101 |
| Reverse: CGTGGTAGGTGGTGTCAGACC | ||
| 16S | Forward: GAACGCTGAAAGAGAAATGA | 216 |
| Reverse: TGTCACCTCTACTCAAAGCTC | ||
| TBP | Forward: CGGGAGCCAAGAGTGAGGA | 51 |
| Reverse: GAGCGTATTTTCTGGCAGCTAAC |
| Gene . | Primer (5′–3′) . | PCR product size (bp) . |
|---|---|---|
| CS | Forward: CCATCGACTCCAACCTGGAC | 51 |
| Reverse: ATCCCAGCATGTTGCTGAAGT | ||
| EF-1A | Forward: CTGGAAGCCAGTGAAAAGATGAC | 51 |
| Reverse: ACGCTCAACCTTCCATCCC | ||
| 18S | Forward: ACCACATCCAAGGAAGGCAG | 51 |
| Reverse: CCGAGTCGGGAGTGGGTAAT | ||
| ND2 | Forward: TAATAGCACAAAATCACCACC | 91 |
| Reverse: GCAAAAAGTAGTACGGCG | ||
| NRF-1 | Forward: TCCAGCAGGTTCAGGTACATGT | 51 |
| Reverse: TCATCATGGACGCTTCGGTAT | ||
| PGC-1α | Forward: CTCCAAATGACCACAAGGGATC | 101 |
| Reverse: CGTGGTAGGTGGTGTCAGACC | ||
| 16S | Forward: GAACGCTGAAAGAGAAATGA | 216 |
| Reverse: TGTCACCTCTACTCAAAGCTC | ||
| TBP | Forward: CGGGAGCCAAGAGTGAGGA | 51 |
| Reverse: GAGCGTATTTTCTGGCAGCTAAC |