Degenerate primers used to obtain partial gene sequences
| Gene . | Primer (5′–3′) . | PCR product size (bp) . |
|---|---|---|
| CS | Forward: GGCGTGCACAAGACCAARTAYTGGGA | 255 |
| Reverse: GGACACGTTGCCGCCYTCRTGRTC | ||
| EF-1A | Forward: CGACATCGCCCTGTGGAARTTYGARAC | 561 |
| Reverse: GATGGCCGCCGATCTTGTANACRTCYTG | ||
| 18S | Forward: ACTGTGGYAATYCYAGAGCTAATACATGC | 400 |
| Reverse: TRYRCTCATTCCRATTACAGGGCC | ||
| NRF-1 | Forward: CAGACCGAGCACATGACCACNATHGARGC | 370 |
| Reverse: TGTCCCACTCGGGTGGTRTAYTCRTC | ||
| PGC-1α | Forward: GGCACTGCACCGAGCTGYTNAARTAYYT | 1200 |
| Reverse: GCAGGGGTGGCCGAARTGYTTRTT | ||
| TBP | Forward: GGAGGAGCAGCAGCGACARCARCARCA | 516 |
| Reverse: GGATCCCACCATGTTCTGGATYTTRAARTC |
| Gene . | Primer (5′–3′) . | PCR product size (bp) . |
|---|---|---|
| CS | Forward: GGCGTGCACAAGACCAARTAYTGGGA | 255 |
| Reverse: GGACACGTTGCCGCCYTCRTGRTC | ||
| EF-1A | Forward: CGACATCGCCCTGTGGAARTTYGARAC | 561 |
| Reverse: GATGGCCGCCGATCTTGTANACRTCYTG | ||
| 18S | Forward: ACTGTGGYAATYCYAGAGCTAATACATGC | 400 |
| Reverse: TRYRCTCATTCCRATTACAGGGCC | ||
| NRF-1 | Forward: CAGACCGAGCACATGACCACNATHGARGC | 370 |
| Reverse: TGTCCCACTCGGGTGGTRTAYTCRTC | ||
| PGC-1α | Forward: GGCACTGCACCGAGCTGYTNAARTAYYT | 1200 |
| Reverse: GCAGGGGTGGCCGAARTGYTTRTT | ||
| TBP | Forward: GGAGGAGCAGCAGCGACARCARCARCA | 516 |
| Reverse: GGATCCCACCATGTTCTGGATYTTRAARTC |
N, R and Y indicate degenerate nucleotides (N=A+C+G+T; R=A+G; Y=C+T)