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Table 2.

Alexa Fluor dyes used to label antibodies against Na+/K+-ATPase, NKCC1a, NCC, NHE3 and CFTR

Alexa FluorEx/Em* (nm)Ex/Em(nm)Pseudocolor
Na+/K+-ATPase 488 495/519 488/505-530 Red 
NKCC1a 430 434/539 405/>530 Blue 
NCC 405 402/421 405/420-480 Cyan 
NHE3 555 555/565 543/560-615 Yellow 
CFTR 647 650/668 633/>650 Green 
Alexa FluorEx/Em* (nm)Ex/Em(nm)Pseudocolor
Na+/K+-ATPase 488 495/519 488/505-530 Red 
NKCC1a 430 434/539 405/>530 Blue 
NCC 405 402/421 405/420-480 Cyan 
NHE3 555 555/565 543/560-615 Yellow 
CFTR 647 650/668 633/>650 Green 
*

Wavelength of maximum excitation and emission of each Alexa Fluor dye

Wavelength of laser excitation and recorded emission for confocal laser-scanning microscopy

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