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Table 2.

Primers used to amplify rainbow trout target genes by real-time RT-PCR

GenePrimer sequencesHybridization temperature (°C)Amplicon size (bp)
EF1α F: 5′-TCCTCTTGGTCGTTTCGCTG-3′ 59 159 
 R: 5′-ACCCGAGGGACATCCTGTG-3′   
GK F: 5′-TGAAGGATCAGAGGTGGGTGATT-3′ 59 253 
 R: 5′-GAAGGTGAAACCCAGAGGAAGC-3′   
PK F: 5′-CCATCGTCGCGGTAACAAGA-3′ 59 158 
 R: 5′-ACATAGGAAAGGCCAGGGGC-3′   
mPEPCK F: 5′-GTTGGTGCTAAAGGGCACAC-3′ 59 149 
 R: 5′-CCCGTCTTCTGATAAGTCCAA-3′   
G6Pase 1 F: 5′-CTCAGTGGCGACAGAAAGG-3′ 55 77 
 R: 5′-TACACAGCAGCATCCAGAGC-3′   
G6Pase 2 F: 5′-TAGCCATCATGCTGACCAAG-3′ 55 82 
 R: 5′-CAGAAGAACGCCCACAGAGT-3′   
FBPase F: 5′-GCTGGACCCTTCCATCGG-3′ 59 182 
 R: 5′-CGACATAACGCCCACCATAGG-3′   
FAS F: 5′-TGATCTGAAGGCCCGTGTCA-3′ 60 161 
 R: 5′-GGGTGACGTTGCCGTGGTAT-3′   
CPT1A F: 5′-TCGATTTTCAAGGGTCTTCG-3′ 55 166 
 R: 5′-CACAACGATCAGCAAACTGG-3′   
CPT1B F: 5′-CCCTAAGCAAAAAGGGTCTTCA-3′ 55 149 
 R: 5′-CATGATGTCACTCCCGACAG-3′   
GenePrimer sequencesHybridization temperature (°C)Amplicon size (bp)
EF1α F: 5′-TCCTCTTGGTCGTTTCGCTG-3′ 59 159 
 R: 5′-ACCCGAGGGACATCCTGTG-3′   
GK F: 5′-TGAAGGATCAGAGGTGGGTGATT-3′ 59 253 
 R: 5′-GAAGGTGAAACCCAGAGGAAGC-3′   
PK F: 5′-CCATCGTCGCGGTAACAAGA-3′ 59 158 
 R: 5′-ACATAGGAAAGGCCAGGGGC-3′   
mPEPCK F: 5′-GTTGGTGCTAAAGGGCACAC-3′ 59 149 
 R: 5′-CCCGTCTTCTGATAAGTCCAA-3′   
G6Pase 1 F: 5′-CTCAGTGGCGACAGAAAGG-3′ 55 77 
 R: 5′-TACACAGCAGCATCCAGAGC-3′   
G6Pase 2 F: 5′-TAGCCATCATGCTGACCAAG-3′ 55 82 
 R: 5′-CAGAAGAACGCCCACAGAGT-3′   
FBPase F: 5′-GCTGGACCCTTCCATCGG-3′ 59 182 
 R: 5′-CGACATAACGCCCACCATAGG-3′   
FAS F: 5′-TGATCTGAAGGCCCGTGTCA-3′ 60 161 
 R: 5′-GGGTGACGTTGCCGTGGTAT-3′   
CPT1A F: 5′-TCGATTTTCAAGGGTCTTCG-3′ 55 166 
 R: 5′-CACAACGATCAGCAAACTGG-3′   
CPT1B F: 5′-CCCTAAGCAAAAAGGGTCTTCA-3′ 55 149 
 R: 5′-CATGATGTCACTCCCGACAG-3′   

R, reverse primer; F, forward primer

GenBank accession no. or sigenae accession no.: EF1α(elongation factor 1α), AF498320; GK (glucokinase), AF135403; PK (pyruvate kinase), AF 246146; mPEPCK (phosphoenolpyruvate carboxykinase, mitochondrial isoform), AF246149; G6Pase 1 (glucose 6-phosphatase 1), tcay0019b.d.18_3.1.s.om.8.1-1693; G6Pase 2 (glucose 6-phosphatase 2), AF120150; FBPase (fructose 1,6-bisphosphatase),AF333188; FAS (fatty acid synthase), tcab0001c.e.06_5.1.s.om.8; CPT1A (carnitine palmitoyltransferase 1 A), AF 327058; CPT1(carnitine palmitoyltransferase 1 B), AF606076

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