Primers used to amplify rainbow trout target genes by conventional RT-PCR
| Gene . | Primer sequences . | Hybridization temperature (°C) . | Amplicon size (bp) . |
|---|---|---|---|
| InsR (c form) | F: 5′-TCATTTGGTGTGGTGCTG-3′ | 56 | 413 |
| R: 5′-CCCGCTTCGTCCCTCTCC-3′ | |||
| HNF4 | F: 5′-TGCAGGTTTAACCGTCAGTG-3′ | 55 | 192 |
| R: 5′-AGAGTTAGTGCTGGGCTGGA-3′ | |||
| AP | F: 5′-ACGAGCCAGGCCACTTCATC-3′ | 60 | 220 |
| R: 5′-TCAGGGCCCATGTGGAAGTT-3′ | |||
| TAT | F: 5′-GTCTTCTGCCTGCCCGCTAC-3′ | 60 | 157 |
| R: 5′-GGTCTAGCTCGTTGCTGTCTTGG-3′ | |||
| STP | F: 5′-CAGCAGCACAGATGGCGTTT-3′ | 60 | 186 |
| R: 5′-GGCCTTTGAGGAGAGCCACA-3′ | |||
| GLUT2 | F: 5′-CCGTCTTTACCATGGTGTCG-3′ | 57 | 222 |
| R: 5′-CCACAATGAACCAGGGGATG-3′ |
| Gene . | Primer sequences . | Hybridization temperature (°C) . | Amplicon size (bp) . |
|---|---|---|---|
| InsR (c form) | F: 5′-TCATTTGGTGTGGTGCTG-3′ | 56 | 413 |
| R: 5′-CCCGCTTCGTCCCTCTCC-3′ | |||
| HNF4 | F: 5′-TGCAGGTTTAACCGTCAGTG-3′ | 55 | 192 |
| R: 5′-AGAGTTAGTGCTGGGCTGGA-3′ | |||
| AP | F: 5′-ACGAGCCAGGCCACTTCATC-3′ | 60 | 220 |
| R: 5′-TCAGGGCCCATGTGGAAGTT-3′ | |||
| TAT | F: 5′-GTCTTCTGCCTGCCCGCTAC-3′ | 60 | 157 |
| R: 5′-GGTCTAGCTCGTTGCTGTCTTGG-3′ | |||
| STP | F: 5′-CAGCAGCACAGATGGCGTTT-3′ | 60 | 186 |
| R: 5′-GGCCTTTGAGGAGAGCCACA-3′ | |||
| GLUT2 | F: 5′-CCGTCTTTACCATGGTGTCG-3′ | 57 | 222 |
| R: 5′-CCACAATGAACCAGGGGATG-3′ |
R, reverse primer; F, forward primer
GenBank accession no. or sigenae accession no.: InsR (c form;insulin receptor), AF062498; HNF4 (hepatic nuclear factor 4),tcbk0002c.b.02_5.1.s.om.8; AP (serum albumin 1 precursor),tcaa0001c.c.01_5.1.s.om.8; TAT (tyrosine aminotransferase),tcay0014b.e.19_3.1.s.om.8; STP (serotransferin precursor),tcay0003b.j.14_3.1.s.om.8; GLUT2 (glucose transporter 2),AF246147