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Table 1.

Primers used to amplify rainbow trout target genes by conventional RT-PCR

GenePrimer sequencesHybridization temperature (°C)Amplicon size (bp)
InsR (c form) F: 5′-TCATTTGGTGTGGTGCTG-3′ 56 413 
 R: 5′-CCCGCTTCGTCCCTCTCC-3′   
HNF4 F: 5′-TGCAGGTTTAACCGTCAGTG-3′ 55 192 
 R: 5′-AGAGTTAGTGCTGGGCTGGA-3′   
AP F: 5′-ACGAGCCAGGCCACTTCATC-3′ 60 220 
 R: 5′-TCAGGGCCCATGTGGAAGTT-3′   
TAT F: 5′-GTCTTCTGCCTGCCCGCTAC-3′ 60 157 
 R: 5′-GGTCTAGCTCGTTGCTGTCTTGG-3′   
STP F: 5′-CAGCAGCACAGATGGCGTTT-3′ 60 186 
 R: 5′-GGCCTTTGAGGAGAGCCACA-3′   
GLUT2 F: 5′-CCGTCTTTACCATGGTGTCG-3′ 57 222 
 R: 5′-CCACAATGAACCAGGGGATG-3′   
GenePrimer sequencesHybridization temperature (°C)Amplicon size (bp)
InsR (c form) F: 5′-TCATTTGGTGTGGTGCTG-3′ 56 413 
 R: 5′-CCCGCTTCGTCCCTCTCC-3′   
HNF4 F: 5′-TGCAGGTTTAACCGTCAGTG-3′ 55 192 
 R: 5′-AGAGTTAGTGCTGGGCTGGA-3′   
AP F: 5′-ACGAGCCAGGCCACTTCATC-3′ 60 220 
 R: 5′-TCAGGGCCCATGTGGAAGTT-3′   
TAT F: 5′-GTCTTCTGCCTGCCCGCTAC-3′ 60 157 
 R: 5′-GGTCTAGCTCGTTGCTGTCTTGG-3′   
STP F: 5′-CAGCAGCACAGATGGCGTTT-3′ 60 186 
 R: 5′-GGCCTTTGAGGAGAGCCACA-3′   
GLUT2 F: 5′-CCGTCTTTACCATGGTGTCG-3′ 57 222 
 R: 5′-CCACAATGAACCAGGGGATG-3′   

R, reverse primer; F, forward primer

GenBank accession no. or sigenae accession no.: InsR (c form;insulin receptor), AF062498; HNF4 (hepatic nuclear factor 4),tcbk0002c.b.02_5.1.s.om.8; AP (serum albumin 1 precursor),tcaa0001c.c.01_5.1.s.om.8; TAT (tyrosine aminotransferase),tcay0014b.e.19_3.1.s.om.8; STP (serotransferin precursor),tcay0003b.j.14_3.1.s.om.8; GLUT2 (glucose transporter 2),AF246147

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