Table 1.

Components of lipid rafts are enriched in the trypanosome flagellum

Molecule Flagellar enrichment
EP Procyclin   1  
GP63   0.9  
PFR   64  
Calflagin Tb24   48  
α-Tubulin   8.8  
Whole cell body   Not detected  
Dehydroergosterol   22  
Molecule Flagellar enrichment
EP Procyclin   1  
GP63   0.9  
PFR   64  
Calflagin Tb24   48  
α-Tubulin   8.8  
Whole cell body   Not detected  
Dehydroergosterol   22  

Flagellar and whole cell preparations were analyzed by western blotting (proteins) or by spectrophotometry (dehydroergosterol). The volume of flagellar preparation necessary to give signal equivalent to 1×106 whole cells was first calculated from standard curves and tabulated as the `raw score'. Values for relative flagellar enrichment were tabulated by normalization to EP procyclin. PFR, calflagin Tb24, dehydroergosterol and tubulin were all found to be enriched in the flagellar fraction relative to procyclin, whereas GP63 was not. WCB, an antigen absent from the flagellar cytoskeleton was not detected in the purified flagella

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