Primers used for real-time PCR
| . | Primer sequence (5′-3′) . | . | |
|---|---|---|---|
| Gene . | Forward . | Reverse . | |
| Mouse | |||
| Cripto | GCCTATGGGATTCCCTTCC | ACAGCGGGATACAGGGACT | |
| Hes1 | ACACCGGACAAACCAAAGAC | CGCCTCTTCTCCATGATAGG | |
| Hes5 | GATGCTCAGTCCCAAGGAGA | AGCTTCAGCTGCTCTATGCTG | |
| Hesr1 | CATGAAGAGAGCTCACCCAGA | CGCCGAACTCAAGTTTCC | |
| Hesr2 | GTGGGGAGCGAGAACAATTA | GTTGTCGGTGAATTGGACCT | |
| Hesr3 | CTGAATTGCGACGATTGGT | GCAAGACCTCAGCTTTCTCC | |
| Nodal | CCAACCATGCCTACATCCA | CACAGCACGTGGAAGGAAC | |
| Sox1 | GTGACATCTGCCCCCATC | GAGGCCAGTCTGGTGTCAG | |
| Wnt1 | TACTGGCACTGACCGCTCT | CTTGGAATCCGTCAACAGGT | |
| Wnt3 | CTCGCTGGCTACCCAATTT | GAGGCCAGAGATGTGTACTGC | |
| β-actin | CTAAGGCCAACCGTGAAAAG | ACCAGAGGCATACAGGGACA | |
| Human | |||
| BF1 | TACTACCGCGAGAACAAGCA | TCACGAAGCACTTGTTGAGG | |
| CHX10 | GCTGGACACCAGCCAGAC | GCAGATTTGGACATTTTTCGAT | |
| CRALBP | AGATCTCAGGAAGATGGTGGAC | GAAGTGGATGGCTTTGAACC | |
| CRX | CACCAGGCTGTGCCCTAC | CTTCCAGCTCCTCCAGTTG | |
| GBX2 | AAAGAGGGCTCGCTGCTC | ATCGCTCTCCAGCGAGAA | |
| HOXB4 | TGGATGCGCAAAGTTCAC | GCTGGACACCAGCCAGAC | |
| IRX3 | AAAAGTTACTCAAGACAGCTTTCCA | GAAATTCCTTCTCCAGCTCCA | |
| MITF | AGAGTCTGAAGCAAGAGCACTG | TGCGGTCATTTATGTTAAATCTTC | |
| NANOG | ATGCCTCACACGGAGACTGT | AGGGCTGTCCTGAATAAGCA | |
| OCT3/4 | GCAAAACCCGGAGGAGGAGTC | CCACATCGGCCTGTGTATATC | |
| PAX6 | TCACCATGGCAAATAACCTG | CAGCATGCAGGAGTATGAGG | |
| RCVRN | TAACGGGACCATCAGCAAG | CCTCGGGAGTGATCATTTTG | |
| RPE65 | CAATGGGTTTCTGATTGTGGA | CCAGTTCTCACGTAAATTGGCTA | |
| RX | GGCAAGGTCAACCTACCAGA | CTTCATGGAGGACACTTCCAG | |
| SIX3 | CCGGAAGAGTTGTCCATGTT | CTCCTCCAGCGTCTCACAG | |
| β-actin | ATTGGCAATGAGCGGTTC | GGATGCCACAGGACTCCA | |
| . | Primer sequence (5′-3′) . | . | |
|---|---|---|---|
| Gene . | Forward . | Reverse . | |
| Mouse | |||
| Cripto | GCCTATGGGATTCCCTTCC | ACAGCGGGATACAGGGACT | |
| Hes1 | ACACCGGACAAACCAAAGAC | CGCCTCTTCTCCATGATAGG | |
| Hes5 | GATGCTCAGTCCCAAGGAGA | AGCTTCAGCTGCTCTATGCTG | |
| Hesr1 | CATGAAGAGAGCTCACCCAGA | CGCCGAACTCAAGTTTCC | |
| Hesr2 | GTGGGGAGCGAGAACAATTA | GTTGTCGGTGAATTGGACCT | |
| Hesr3 | CTGAATTGCGACGATTGGT | GCAAGACCTCAGCTTTCTCC | |
| Nodal | CCAACCATGCCTACATCCA | CACAGCACGTGGAAGGAAC | |
| Sox1 | GTGACATCTGCCCCCATC | GAGGCCAGTCTGGTGTCAG | |
| Wnt1 | TACTGGCACTGACCGCTCT | CTTGGAATCCGTCAACAGGT | |
| Wnt3 | CTCGCTGGCTACCCAATTT | GAGGCCAGAGATGTGTACTGC | |
| β-actin | CTAAGGCCAACCGTGAAAAG | ACCAGAGGCATACAGGGACA | |
| Human | |||
| BF1 | TACTACCGCGAGAACAAGCA | TCACGAAGCACTTGTTGAGG | |
| CHX10 | GCTGGACACCAGCCAGAC | GCAGATTTGGACATTTTTCGAT | |
| CRALBP | AGATCTCAGGAAGATGGTGGAC | GAAGTGGATGGCTTTGAACC | |
| CRX | CACCAGGCTGTGCCCTAC | CTTCCAGCTCCTCCAGTTG | |
| GBX2 | AAAGAGGGCTCGCTGCTC | ATCGCTCTCCAGCGAGAA | |
| HOXB4 | TGGATGCGCAAAGTTCAC | GCTGGACACCAGCCAGAC | |
| IRX3 | AAAAGTTACTCAAGACAGCTTTCCA | GAAATTCCTTCTCCAGCTCCA | |
| MITF | AGAGTCTGAAGCAAGAGCACTG | TGCGGTCATTTATGTTAAATCTTC | |
| NANOG | ATGCCTCACACGGAGACTGT | AGGGCTGTCCTGAATAAGCA | |
| OCT3/4 | GCAAAACCCGGAGGAGGAGTC | CCACATCGGCCTGTGTATATC | |
| PAX6 | TCACCATGGCAAATAACCTG | CAGCATGCAGGAGTATGAGG | |
| RCVRN | TAACGGGACCATCAGCAAG | CCTCGGGAGTGATCATTTTG | |
| RPE65 | CAATGGGTTTCTGATTGTGGA | CCAGTTCTCACGTAAATTGGCTA | |
| RX | GGCAAGGTCAACCTACCAGA | CTTCATGGAGGACACTTCCAG | |
| SIX3 | CCGGAAGAGTTGTCCATGTT | CTCCTCCAGCGTCTCACAG | |
| β-actin | ATTGGCAATGAGCGGTTC | GGATGCCACAGGACTCCA | |