The Company of Biologists

Table 2.

Fluorescence intensity of Plk4 at the centrosome in HCT116 cells

HCT116 cells	HU treatment^¹	Counted cells^²	Fluorescence intensity^³  Abnormal centrosomes
HCT116 cells	HU treatment^¹	Counted cells^²			-	+
p53^+/+	+	9	318.5	313.7
p53^+/+	+	82	496.5	470.0
p53^+/+	-	13	543.9	467.6
p53^-/-	+	21	669.0	644.0

HCT116 cells	HU treatment^¹	Counted cells^²	Fluorescence intensity^³  Abnormal centrosomes
HCT116 cells	HU treatment^¹	Counted cells^²			-	+
p53^+/+	+	9	318.5	313.7
p53^+/+	+	82	496.5	470.0
p53^+/+	-	13	543.9	467.6
p53^-/-	+	21	669.0	644.0

Cells were treated with or without hydroxyurea for 3 days.

Numbers of paired cells (+/- γ-tubulin-specific abnormal centrosomes) counted.

Mean intensity (arbitrary units).