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Table 1.

Fumarase- and aconitase-MTS-exchange-mutants

Plasmid Mutant protein MPII Score Hmax μH§ MTS sequence (3′ to 5′) Distribution Mito vs Cyt
WT-FUM1  WT-Fum1   0.9265   4.56   8.38   MLRFTNCSCKTFVKSSYKLNIRRM*NSSFRT   30:70  
A   AcoMTS-Fum1   0.9964   3.02   5.02  MLSARSAIKRPIVRGLATVSSFRT   40:60  
B   Hsp60MTS-Fum1   0.9995   4.96   8.53  MLRSSVVRSRATLRPLLRRAYSSSFRT   58:42  
WT-ACO1  WT-Aco1   0.9950   5.01   6.23   MLSARSAIKRPIVRGL * ATV   94:6  
C   FumMTS-Aco1   0.9653   4.56   8.3  MLRFTNCSCKTFVKSSYKLNIRRMNTV   50:50  
Plasmid Mutant protein MPII Score Hmax μH§ MTS sequence (3′ to 5′) Distribution Mito vs Cyt
WT-FUM1  WT-Fum1   0.9265   4.56   8.38   MLRFTNCSCKTFVKSSYKLNIRRM*NSSFRT   30:70  
A   AcoMTS-Fum1   0.9964   3.02   5.02  MLSARSAIKRPIVRGLATVSSFRT   40:60  
B   Hsp60MTS-Fum1   0.9995   4.96   8.53  MLRSSVVRSRATLRPLLRRAYSSSFRT   58:42  
WT-ACO1  WT-Aco1   0.9950   5.01   6.23   MLSARSAIKRPIVRGL * ATV   94:6  
C   FumMTS-Aco1   0.9653   4.56   8.3  MLRFTNCSCKTFVKSSYKLNIRRMNTV   50:50  

MitoProtII score (Claros et al., 1997)

Maximal hydrophobicity of the hydrophobic face of the helical structure (von Heijne, 1986)

§

Hydrophobic moment (von Heijne, 1986)

Percentage of mitochondrial and cytosolic fractions. Modifications of the amino acid sequences are in bold letters

*

, MPP cleavage site

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