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Table 2.

Increased lateral mobility of GFP-tagged TCs in M3/18 cells expressing DRM-tagged spastin or a CLIMP-63 deletion mutant lacking the microtubule-binding domain

Calculated TC assemblies*
Cell line GFP-tagged reporter DRM-tagged construct Deff (μm2/s) ± s.d. nP(T⩽t) Radius (nm) n of TCs
BHK-21   LBR-GFP   –   0.391±0.120   15   –   0.5   –  
   p63-DRM   0.391±0.045   6   0.999   0.5   –  
M3/18   GFP-Dad1   –   0.060±0.009   34   –   338   5867  
   DRM-wtSPG4   0.125±0.024   7   <0.001   11   7  
   DRM-NK#1   0.058±0.011   9   0.612   423   9196  
   p63-DRM   0.063±0.007   7   0.672   248   3154  
   ΔLp63-DRM   0.059±0.012   18   0.643   350   8763  
   DRM-ΔCp63   0.088±0.021   14   0.001   93   93  
   ΔTLp63-DRM   0.098±0.013   5   0.001   37   37  
   DRM   0.053±0.010   7   0.182   –   –  
Calculated TC assemblies*
Cell line GFP-tagged reporter DRM-tagged construct Deff (μm2/s) ± s.d. nP(T⩽t) Radius (nm) n of TCs
BHK-21   LBR-GFP   –   0.391±0.120   15   –   0.5   –  
   p63-DRM   0.391±0.045   6   0.999   0.5   –  
M3/18   GFP-Dad1   –   0.060±0.009   34   –   338   5867  
   DRM-wtSPG4   0.125±0.024   7   <0.001   11   7  
   DRM-NK#1   0.058±0.011   9   0.612   423   9196  
   p63-DRM   0.063±0.007   7   0.672   248   3154  
   ΔLp63-DRM   0.059±0.012   18   0.643   350   8763  
   DRM-ΔCp63   0.088±0.021   14   0.001   93   93  
   ΔTLp63-DRM   0.098±0.013   5   0.001   37   37  
   DRM   0.053±0.010   7   0.182   –   –  
*

Since microtubules remain intact in all experiments, except in the one where FRAP was done on the cells expressing spastin (DRW-wtSPG4), the calculated radius values and the numbers (n) of TC assemblies are overestimated because of interactions of the TCs with microtubules. See also footnote to Table 1 and see Materials and Methods on the calculation of the sizes of TC assemblies

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