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Table 1.

The lateral mobility of TCs in M3/18 cells is increased by treatments that inhibit protein synthesis or affect microtubule integrity

Calculated TC assemblies*
Cell line GFP-tagged reporter Treatment Deff (μm2/s) ± s.d. nP(T⩽t) Radius (nm) n of TCs
BHK-21   LBR-GFP   –   0.391±0.120   15   –   0.5   –  
   CHX   0.386±0.060   7   0.907   0.5   –  
   NC   0.385±0.154   17   0.910   0.5   –  
M3/18   GFP-Dad1   –   0.060±0.009   34   –   338  5867 
   CHX   0.070±0.023   12   0.275   133  912 
   PU   0.165±0.045   14   <0.001   5   1.5  
   NaCl   0.144±0.051   12   0.008   8   3  
   CHX + NaCl   0.073±0.012   8   0.056   106  516 
   LB   0.061±0.013   10   0.950   304  4738 
   NC   0.104±0.026   25   <0.001   21   24  
   NC + PU   0.158±0.043   10   <0.001   6   2  
   Taxol   0.079±0.019   8   0.017   71  255 
Calculated TC assemblies*
Cell line GFP-tagged reporter Treatment Deff (μm2/s) ± s.d. nP(T⩽t) Radius (nm) n of TCs
BHK-21   LBR-GFP   –   0.391±0.120   15   –   0.5   –  
   CHX   0.386±0.060   7   0.907   0.5   –  
   NC   0.385±0.154   17   0.910   0.5   –  
M3/18   GFP-Dad1   –   0.060±0.009   34   –   338  5867 
   CHX   0.070±0.023   12   0.275   133  912 
   PU   0.165±0.045   14   <0.001   5   1.5  
   NaCl   0.144±0.051   12   0.008   8   3  
   CHX + NaCl   0.073±0.012   8   0.056   106  516 
   LB   0.061±0.013   10   0.950   304  4738 
   NC   0.104±0.026   25   <0.001   21   24  
   NC + PU   0.158±0.043   10   <0.001   6   2  
   Taxol   0.079±0.019   8   0.017   71  255 

NC, nocodazole; CHX, cycloheximide; PU, puromycin; LB, lantrunculin B

*

, Calculation of radii of TC assemblies are based on the diffusion constants (Deff) as determined by FRAP. The equation used to calculate these radii takes into account only friction of the transmembrane domains with the lipid bilayer

, Radius values and number (n) of TCs in TC assemblies, obtained under experimental conditions where microtubules remained intact. Radius values are therefore expected to be much higher than the actual size of the TC assemblies owing to the contribution of microtubules that interfere with the free diffusion TCs. See also Materials and Methods for a more detailed explanation related to these calculations

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