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Table 3.

Effects of RNAi of specific actin subfamilies on cell shape and various behavioral and physiological aspects

Actin subfamily	Cell shape	Swimming behavior	De-/hyperpolarisation response	Exocytosis	Phagocytosis^{^†}	Contractile vacuole cycle	Cyclosis
actin1-2	Normal	Normal	Normal	100%	100%	Normal	Normal
actin1-6	Normal	Normal	Normal	100%	100%	Normal	Normal
actin1-9	Normal	Normal	Normal	100%	100%	Normal	Normal
actin2	Normal	Slower	Normal	100%	30%	Normal	Normal
actin2-1 (133bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin2-1 (430bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3-1 (487bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3-1/3-2	Normal	Slower	Normal	100%	30%	Normal	Normal
actin4	Normal	Normal	Normal	100%	30%	Normal	Normal
	boomerang^{^*}	Circles	Normal	<10%	0%	Normal	Normal
actin5	Normal	Normal	Normal	100%	100%	Normal	Normal
actin6	Normal	Normal	Normal	100%	100%	Normal	Normal
actin7	dolphin^{^*}	Normal	Normal	100%	70%	Normal	Normal
actin8	Normal	Normal	Normal	100%	100%	Normal	Normal
actin9	Normal	Normal	Normal	100%	70%	Normal^{^‡}	Normal
	triangle^{^*}	Circles	Normal	100%	10%	Prolonged^{^‡}	Accelerated

Actin subfamily	Cell shape	Swimming behavior	De-/hyperpolarisation response	Exocytosis	Phagocytosis^{^†}	Contractile vacuole cycle	Cyclosis
actin1-2	Normal	Normal	Normal	100%	100%	Normal	Normal
actin1-6	Normal	Normal	Normal	100%	100%	Normal	Normal
actin1-9	Normal	Normal	Normal	100%	100%	Normal	Normal
actin2	Normal	Slower	Normal	100%	30%	Normal	Normal
actin2-1 (133bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin2-1 (430bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3-1 (487bp)	Normal	Slower	Normal	100%	30%	Normal	Normal
actin3-1/3-2	Normal	Slower	Normal	100%	30%	Normal	Normal
actin4	Normal	Normal	Normal	100%	30%	Normal	Normal
	boomerang^{^*}	Circles	Normal	<10%	0%	Normal	Normal
actin5	Normal	Normal	Normal	100%	100%	Normal	Normal
actin6	Normal	Normal	Normal	100%	100%	Normal	Normal
actin7	dolphin^{^*}	Normal	Normal	100%	70%	Normal	Normal
actin8	Normal	Normal	Normal	100%	100%	Normal	Normal
actin9	Normal	Normal	Normal	100%	70%	Normal^{^‡}	Normal
	triangle^{^*}	Circles	Normal	100%	10%	Prolonged^{^‡}	Accelerated

For the subfamilies act2 and act3, constructs of different length were designed. Within each subfamily, the different constructs showed the same effect on the cell, excluding silencing variation owing to the length of the introduced dsRNA or cross-silencing of other subfamilies. Gene silencing of members of the actin1 subfamily or act5, act6 or act8 had no effect on any of the examined aspects. For more details, see text

See Fig. 8

†

See Fig. 9

‡

See Fig. 10

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