Localization of the different actin isoforms
| . | GFP localization . | . | . | . | . | . | . | . | . | AB localization . | . | . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | actin1-2 . | actin1-4 . | actin1-6 . | actin1-9 . | actin2-1 . | actin3-1 . | actin5-1 . | actin6-1 . | actin8-1 . | actin1-1* . | actin4-1 . | actin5-1 . | ||||||||||
| Cortex | — | — | — | — | — | + | + | — | + | + | + | + | ||||||||||
| Oral cavity | — | — | — | — | — | — | + | — | + | + | + | + | ||||||||||
| Oral filaments | — | — | — | — | — | — | + | — | + | — | — | + | ||||||||||
| Phagosomes | + | — | — | + | — | + | + | — | + | + | — | + | ||||||||||
| Cilia | — | — | — | — | + | + | — | — | — | + | + | + | ||||||||||
| Cytosolic compartment | — | + | + | — | + | + | — | + | — | — | — | — | ||||||||||
| Cleavage furrow | — | — | — | — | — | — | — | — | — | — | + | — | ||||||||||
| . | GFP localization . | . | . | . | . | . | . | . | . | AB localization . | . | . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | actin1-2 . | actin1-4 . | actin1-6 . | actin1-9 . | actin2-1 . | actin3-1 . | actin5-1 . | actin6-1 . | actin8-1 . | actin1-1* . | actin4-1 . | actin5-1 . | ||||||||||
| Cortex | — | — | — | — | — | + | + | — | + | + | + | + | ||||||||||
| Oral cavity | — | — | — | — | — | — | + | — | + | + | + | + | ||||||||||
| Oral filaments | — | — | — | — | — | — | + | — | + | — | — | + | ||||||||||
| Phagosomes | + | — | — | + | — | + | + | — | + | + | — | + | ||||||||||
| Cilia | — | — | — | — | + | + | — | — | — | + | + | + | ||||||||||
| Cytosolic compartment | — | + | + | — | + | + | — | + | — | — | — | — | ||||||||||
| Cleavage furrow | — | — | — | — | — | — | — | — | — | — | + | — | ||||||||||
For the isoforms act1-2, act1-6, act1-9, act2-1, act3-1, act5-1, act6-1 and act8-1, GFP constructs were used to transform cells and to analyze different localization of the GFP-fusion proteins in descendants. Cells transformed with a GFP-act4-1 construct did not divide and were too instable for localization studies. Therefore, specific antibodies were raised against act4-1. In addition, antibodies against act5-1 were raised. Anti-act5-1 antibodies labeled the same structures as seen with GFP-act5-1 except for the labeling of the cilia. The GFP-signal might not be detected because of the fast movement of cilia, in contrast to immunodetection
As the actin isoforms act1-1, act1-2 and act1-3 are identical at the amino acid level (I.M.S., J.M., C.R., E. Wagner, H.P. and R.K., unpublished), the polyclonal antibodies against act1-1 will also recognize those isoforms and probably also peptides from further members of the act1 subfamily and possibly also of other subfamilies (Kissmehl et al., 2004)